MBL2 coding genotype frequencies and baseline MBL levels in donors and recipients
| MBL2 genotype . | Mannan binding . | C4-deposition levels . | ||||||
|---|---|---|---|---|---|---|---|---|
| Donor . | Recipient . | P . | Donor . | Recipient . | P . | |||
| n . | Mean plus or minus SD, μg/mL (range) . | n . | Mean plus or minus SD, μg/mL (range) . | Mean plus or minus SD, U/mL (range) . | Mean plus or minus SD, U/mL (range) . | |||
| A/A | 85 | 2.53 ± 1.36 (0.40-7.4) | 92 | 3.69 ± 1.81 (0.54-10.25) | <.001 | 0.58 ± 0.36 (0-2.25) | 1.01 ± 0.70 (0.12-3.81) | <.001 |
| A/O | 44 | 0.58 ± 1.023 (0-6.59) | 36 | 0.68 ± 0.78 (0-3.10) | NS | 0.14 ± 0.17 (0.01-0.95) | 0.21 ± 0.22 (0.02-0.87) | .095 |
| A/B | 30 | 0.52 ± 1.18 (0-6.59) | 21 | 0.39 ± 0.32 (0-1.01) | NS | 0.11 ± 0.17 (0.01-0.95) | 0.12 ± 0.07 (0.02-0.24) | NS |
| A/C | 1 | 1.47 | 3 | 0.03 ± 0.04 (0-0.08) | 0.23 | 0.06 ± 0.03 (0.03-0.08) | ||
| A/D | 13 | 0.65 ± 0.55 (0-1.89) | 12 | 1.35 ± 1.00 (0-3.10) | .038 | 0.18 ± 0.17 (0.03-0.61) | 0.41 ± 0.28 (0.03-0.87) | .019 |
| O/O | 7 | 0 ± 0 (0-0) | 8 | 0 ± 0 (0-0) | NS | 0.07 ± 0.07 (0.0-0.19) | 0.04 ± 0.02 (0.01-0.07) | NS |
| YA/YA | 50 | 3.11 ± 1.35 (0.89-7.39) | 54 | 4.10 ± 1.64 (1.46-8.57) | .001 | 0.70 ± 0.40 (0-2.25) | 1.00 ± 0.64 (0.20-3.64) | .005 |
| YA/XA | 32 | 1.88 ± 0.91 (0.51-4.84) | 32 | 3.49 ± 1.81 (1.16-10.25) | <.001 | 0.44 ± 0.22 (0.03-0.92) | 1.15 ± 0.79 (0.30-3.81) | <.001 |
| YA/O | 33 | 0.74 ± 1.14 (0-6.59) | 26 | 0.87 ± 0.82 (0.0-3.1) | NS | 0.16 ± 0.18 (0.03-0.95) | 0.35 ± 0.23 (0.04-0.87) | .092 |
| XA/XA | 3 | 0.61 ± 0.21 (0.40-0.81) | 6 | 1.20 ± 0.77 (0.54-2.64) | NS | 0.15 ± 0.04 (0.13-0.2) | 0.30 ± 0.25 (0.12-0.78) | NS |
| XA/O | 11 | 0.10 ± 0.16 (0-0.51 | 10 | 0.18 ± 0.40 (0-1.29) | NS | 0.06 ± 0.03 (0.01-0.10) | 0.09 ± 0.17 (0.02-0.56) | NS |
| HYA/HYA | 15 | 3.11 ± 1.37 (1.33-6.8) | 20 | 3.96 ± 1.46 (1.97-6.78) | .082 | 0.75 ± 0.53 (0-2.25) | 0.87 ± 0.43 (0.27-1.76) | NS |
| HYA/LYA | 30 | 3.18 ± 1.40 (0.89-7.40) | 30 | 4.24 ± 1.79 (1.46-8.57) | .013 | 0.70 ± 0.33 (0.11-1.58) | 1.11 ± 0.79 (0.20-3.64) | .011 |
| HYA/LXA | 19 | 1.92 ± 0.92 (0.82-4.84) | 16 | 3.33 ± 1.30 (1.16-5.37) | .001 | 0.46 ± 0.22 (0.11-0.92) | 1.20 ± 0.86 (0.36-3.81) | .001 |
| HYA/B | 12 | 0.52 ± 0.32 (0.03-1.26) | 12 | 0.52 ± 0.34 (0-1.01) | NS | 0.12 ± 0.06 (0.03-0.22) | 0.14 ± 0.07 (0.04-0.23) | NS |
| HYA/C | 1 | 1.45 | 0 | 0.233 | ||||
| HYA/D | 9 | 0.84 ± 0.54 (0-1.89) | 4 | 1.83 ± 0.93 (0.91-3.10) | .032 | 0.23 ± 0.18 (0.03-0.61) | 0.50 ± 0.27 (0.26-0.87) | .057 |
| LYA/LYA | 6 | 2.50 ± 1.02 (1.01-3.92) | 5 | 3.51 ± 1.43 (1.91-5.68) | NS | 0.56 ± 0.33 (0.29-1.20) | 0.84 ± 0.09 (0.73-0.97) | .100 |
| LYA/LXA | 14 | 1.77 ± 0.91 (0.51-3.83) | 17 | 3.66 ± 2.21 (1.48-10.25) | .006 | 0.41 ± 0.23 (0.04-0.78) | 1.1 ± 0.74 (0.30-3.36) | .003 |
| LYA/B | 11 | 0.83 ± 1.92 (0.06-6.59 | 5 | 0.34 ± 0.15 (0.19-0.55) | NS | 0.15 ± 0.27 (0.03-0.95) | 0.12 ± 0.08 (0.05-0.24) | NS |
| LYA/D | 0 | 5 | 1.48 ± 1.00 (0-2.43) | 0.46 ± 0.28 (0.05-0.80 | ||||
| LXA/LXA | 3 | 0.61 ± 0.21 (0.40-0.81) | 6 | 1.2 ± 0.77 (0.54-2.64) | NS | 0.15 ± 0.04 (0.13-0.20) | 0.30 ± 0.25 (0.12-0.78) | NS |
| LXA/B | 7 | 0.04 ± 0.06 (0.0-0.16) | 5 | 0.05 ± 0.11 (0-0.24) | NS | 0.05 ± 0.03 (0.01-0.08) | 0.04 ± 0.01 (0.02-0.06) | NS |
| LXA/C | 0 | 2 | 0.04 ± 0.06 (0-0.08) | 0.05 ± 0.04 (0.03-0.08) | ||||
| LXA/D | 4 | 0.22 ± 0.22 (0.0-0.51) | 3 | 0.50 ± 0.69 (0.01-1.29) | NS | 0.07 ± 0.03 (0.04-0.10) | 0.211 ± 0.31 (0.03-0.56) | NS |
| MBL2 genotype . | Mannan binding . | C4-deposition levels . | ||||||
|---|---|---|---|---|---|---|---|---|
| Donor . | Recipient . | P . | Donor . | Recipient . | P . | |||
| n . | Mean plus or minus SD, μg/mL (range) . | n . | Mean plus or minus SD, μg/mL (range) . | Mean plus or minus SD, U/mL (range) . | Mean plus or minus SD, U/mL (range) . | |||
| A/A | 85 | 2.53 ± 1.36 (0.40-7.4) | 92 | 3.69 ± 1.81 (0.54-10.25) | <.001 | 0.58 ± 0.36 (0-2.25) | 1.01 ± 0.70 (0.12-3.81) | <.001 |
| A/O | 44 | 0.58 ± 1.023 (0-6.59) | 36 | 0.68 ± 0.78 (0-3.10) | NS | 0.14 ± 0.17 (0.01-0.95) | 0.21 ± 0.22 (0.02-0.87) | .095 |
| A/B | 30 | 0.52 ± 1.18 (0-6.59) | 21 | 0.39 ± 0.32 (0-1.01) | NS | 0.11 ± 0.17 (0.01-0.95) | 0.12 ± 0.07 (0.02-0.24) | NS |
| A/C | 1 | 1.47 | 3 | 0.03 ± 0.04 (0-0.08) | 0.23 | 0.06 ± 0.03 (0.03-0.08) | ||
| A/D | 13 | 0.65 ± 0.55 (0-1.89) | 12 | 1.35 ± 1.00 (0-3.10) | .038 | 0.18 ± 0.17 (0.03-0.61) | 0.41 ± 0.28 (0.03-0.87) | .019 |
| O/O | 7 | 0 ± 0 (0-0) | 8 | 0 ± 0 (0-0) | NS | 0.07 ± 0.07 (0.0-0.19) | 0.04 ± 0.02 (0.01-0.07) | NS |
| YA/YA | 50 | 3.11 ± 1.35 (0.89-7.39) | 54 | 4.10 ± 1.64 (1.46-8.57) | .001 | 0.70 ± 0.40 (0-2.25) | 1.00 ± 0.64 (0.20-3.64) | .005 |
| YA/XA | 32 | 1.88 ± 0.91 (0.51-4.84) | 32 | 3.49 ± 1.81 (1.16-10.25) | <.001 | 0.44 ± 0.22 (0.03-0.92) | 1.15 ± 0.79 (0.30-3.81) | <.001 |
| YA/O | 33 | 0.74 ± 1.14 (0-6.59) | 26 | 0.87 ± 0.82 (0.0-3.1) | NS | 0.16 ± 0.18 (0.03-0.95) | 0.35 ± 0.23 (0.04-0.87) | .092 |
| XA/XA | 3 | 0.61 ± 0.21 (0.40-0.81) | 6 | 1.20 ± 0.77 (0.54-2.64) | NS | 0.15 ± 0.04 (0.13-0.2) | 0.30 ± 0.25 (0.12-0.78) | NS |
| XA/O | 11 | 0.10 ± 0.16 (0-0.51 | 10 | 0.18 ± 0.40 (0-1.29) | NS | 0.06 ± 0.03 (0.01-0.10) | 0.09 ± 0.17 (0.02-0.56) | NS |
| HYA/HYA | 15 | 3.11 ± 1.37 (1.33-6.8) | 20 | 3.96 ± 1.46 (1.97-6.78) | .082 | 0.75 ± 0.53 (0-2.25) | 0.87 ± 0.43 (0.27-1.76) | NS |
| HYA/LYA | 30 | 3.18 ± 1.40 (0.89-7.40) | 30 | 4.24 ± 1.79 (1.46-8.57) | .013 | 0.70 ± 0.33 (0.11-1.58) | 1.11 ± 0.79 (0.20-3.64) | .011 |
| HYA/LXA | 19 | 1.92 ± 0.92 (0.82-4.84) | 16 | 3.33 ± 1.30 (1.16-5.37) | .001 | 0.46 ± 0.22 (0.11-0.92) | 1.20 ± 0.86 (0.36-3.81) | .001 |
| HYA/B | 12 | 0.52 ± 0.32 (0.03-1.26) | 12 | 0.52 ± 0.34 (0-1.01) | NS | 0.12 ± 0.06 (0.03-0.22) | 0.14 ± 0.07 (0.04-0.23) | NS |
| HYA/C | 1 | 1.45 | 0 | 0.233 | ||||
| HYA/D | 9 | 0.84 ± 0.54 (0-1.89) | 4 | 1.83 ± 0.93 (0.91-3.10) | .032 | 0.23 ± 0.18 (0.03-0.61) | 0.50 ± 0.27 (0.26-0.87) | .057 |
| LYA/LYA | 6 | 2.50 ± 1.02 (1.01-3.92) | 5 | 3.51 ± 1.43 (1.91-5.68) | NS | 0.56 ± 0.33 (0.29-1.20) | 0.84 ± 0.09 (0.73-0.97) | .100 |
| LYA/LXA | 14 | 1.77 ± 0.91 (0.51-3.83) | 17 | 3.66 ± 2.21 (1.48-10.25) | .006 | 0.41 ± 0.23 (0.04-0.78) | 1.1 ± 0.74 (0.30-3.36) | .003 |
| LYA/B | 11 | 0.83 ± 1.92 (0.06-6.59 | 5 | 0.34 ± 0.15 (0.19-0.55) | NS | 0.15 ± 0.27 (0.03-0.95) | 0.12 ± 0.08 (0.05-0.24) | NS |
| LYA/D | 0 | 5 | 1.48 ± 1.00 (0-2.43) | 0.46 ± 0.28 (0.05-0.80 | ||||
| LXA/LXA | 3 | 0.61 ± 0.21 (0.40-0.81) | 6 | 1.2 ± 0.77 (0.54-2.64) | NS | 0.15 ± 0.04 (0.13-0.20) | 0.30 ± 0.25 (0.12-0.78) | NS |
| LXA/B | 7 | 0.04 ± 0.06 (0.0-0.16) | 5 | 0.05 ± 0.11 (0-0.24) | NS | 0.05 ± 0.03 (0.01-0.08) | 0.04 ± 0.01 (0.02-0.06) | NS |
| LXA/C | 0 | 2 | 0.04 ± 0.06 (0-0.08) | 0.05 ± 0.04 (0.03-0.08) | ||||
| LXA/D | 4 | 0.22 ± 0.22 (0.0-0.51) | 3 | 0.50 ± 0.69 (0.01-1.29) | NS | 0.07 ± 0.03 (0.04-0.10) | 0.211 ± 0.31 (0.03-0.56) | NS |
MBL2 genotypic data have been shown as coding genotypes (A indicates coding wild-type; O, coding mutation; and B, C, and D, each of the coding mutations), haplotypes of the MBL2 −221 promoter polymorphism (X/Y) and coding genotype (A or O), and full promoter-coding MBL2 haplotypes, where −550 H/L represents the second promoter polymorphism, and HYA, LYA, and LXA result in high, intermediate, and low levels of MBL, respectively. MBL levels were measured by 2 assays: binding of MBL to solid phase mannan (Mannan binding) and complement fixation (C4-deposition). P values report comparisons of MBL levels for each genotype or haplotype between donors and recipients.
NS indicates not significant.