Overview of diseased mice
| Mouse ID, by type of leukemia/genotype . | Sex . | Age, mo . | Leukocytes,×103/mm3 . | Spleen weight, g . | Liver weight, g . | SKY . | CGH . | Loss of Nf1 WT transcript/WT allele . |
|---|---|---|---|---|---|---|---|---|
| MPD-like myeloid leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 4121 | M | 10.4 | ND | 0.28 | 1.6 | ND | ND | ND |
| 4450 | M | 11.8 | 27.5 | 0.36 | 1.75 | ND | ND | −/− |
| 4266 | M | 12.1 | 28.1 | 0.55 | 2.17 | ND | ND | −/− |
| 4281 | M | 12.1 | 27.6 | 0.31 | 2.54 | ND | Normal | −/− |
| 4926 | M | 8.2 | 66.5 | 0.72 | 2.68 | No CAs | Normal | −/− |
| 4928 | M | 8.2 | 68.5 | 0.9 | 2.78 | No CAs | Normal | −/− |
| 4267 | M | 11.3 | ND | 0.41 | 3.12 | ND | ND | −/− |
| 4767 | F | 9.4 | 27.9 | 0.5 | 1.56 | ND | ND | −/ND |
| 4271 | F | 10.7 | 20.9 | 0.27 | 1.7 | No CAs | Normal | ND |
| 4708 | F | 15.0 | 34.7 | 0.3 | 1.92 | ND | ND | (+)/(+) |
| 4427 | F | 14.7 | 46.4 | 0.52 | 4.41 | ND | ND | +/+ |
| Median | 11.3 | 28.1 | 0.4 | 2.2 | 0 of 3 | 0 of 4 | 2 of 9 | |
| Myelomonocytic leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 4447 | M | 10.2 | 195 | 1.13 | 3.53 | ND | ND | ND |
| 4448 | M | 8.5 | 7.6 | 0.81 | 1.91 | No CAs | ND | ND |
| 4757 | M | 9.8 | 38 | 0.78 | 3.54 | ND | 41; XY; +X | (+)/(+) |
| 3761 | M | 12.2 | 225 | 1.09 | 5.16 | No CAs | Normal | (+)/(+) |
| 4273 | F | 10.2 | 74 | 0.55 | 1.4 | ND | ND | ND |
| 4122 | F | 11.0 | 480 | 0.57 | 2 | No CAs | Normal | ND |
| Median | 10.2 | 134.5 | 0.8 | 2.8 | 0 of 4 | 1 of 3 | 2 of 2 | |
| Lymphatic leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 3782 | M | 12.3 | 826 | 0.7 | 2.87 | ND | ND | ND |
| 4430 | F | 14.9 | 675 | 0.54 | 3.04 | ND | Gain 9A | +/+ |
| 4115 | F | 10.8 | 76.3 | 0.89 | 3.36 | ND | 42, XX, +14, +17 | (+)/(+) |
| Median | 12.3 | 750.5 | 0.7 | 3.0 | ND | 2 of 2 | 2 of 2 | |
| Biphenotypic leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 4765 | M | 10.7 | 1148 | 0.67 | 5.57 | ND | ND | (+)/(?) |
| 4453 | F | 11.2 | 568 | 0.87 | 4.29 | ND | 39, X, -X | +/+ |
| Median | 11.8 | 858.0 | 0.8 | 4.9 | ND | 1 of 1 | 2 of 2 | |
| Leukemia without maturation/Icsbp−/−Nf1+/− | ||||||||
| 4289 | F | 13.6 | 773 | 0.51 | 2.73 | ND | Normal | ND |
| Lymphoma/Icsbp−/−Nf1+/− | ||||||||
| 3529 | M | 11.1 | ND | ND | ND | ND | ND | ND |
| MPD-like myeloid leukemia/Icsbp−/−Nf1+/+ | ||||||||
| 3502 | M | 17.4 | 70.1 | 0.32 | 1.74 | ND | ND | ND |
| 4922 | F | 12 | 30.9 | 0.29 | 2.28 | ND | ND | ND |
| Lymphatic leukemia/ Icsbp−/−Nf1+/+ | ||||||||
| 4921 | F | 7.1 | 476 | 1.2 | 4.44 | ND | ND | ND |
| Leukemia without maturation/Icsbp−/−Nf1+/+ | ||||||||
| 4435 | M | 14.2 | 287 | ND | ND | ND | ND | ND |
| Myelomonocytic leukemia/Icsbp+/+Nf1+/− | ||||||||
| 4712 | F | 8.4 | 58.5 | 1.96 | 3.04 | ND | ND | −/− |
| Mouse ID, by type of leukemia/genotype . | Sex . | Age, mo . | Leukocytes,×103/mm3 . | Spleen weight, g . | Liver weight, g . | SKY . | CGH . | Loss of Nf1 WT transcript/WT allele . |
|---|---|---|---|---|---|---|---|---|
| MPD-like myeloid leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 4121 | M | 10.4 | ND | 0.28 | 1.6 | ND | ND | ND |
| 4450 | M | 11.8 | 27.5 | 0.36 | 1.75 | ND | ND | −/− |
| 4266 | M | 12.1 | 28.1 | 0.55 | 2.17 | ND | ND | −/− |
| 4281 | M | 12.1 | 27.6 | 0.31 | 2.54 | ND | Normal | −/− |
| 4926 | M | 8.2 | 66.5 | 0.72 | 2.68 | No CAs | Normal | −/− |
| 4928 | M | 8.2 | 68.5 | 0.9 | 2.78 | No CAs | Normal | −/− |
| 4267 | M | 11.3 | ND | 0.41 | 3.12 | ND | ND | −/− |
| 4767 | F | 9.4 | 27.9 | 0.5 | 1.56 | ND | ND | −/ND |
| 4271 | F | 10.7 | 20.9 | 0.27 | 1.7 | No CAs | Normal | ND |
| 4708 | F | 15.0 | 34.7 | 0.3 | 1.92 | ND | ND | (+)/(+) |
| 4427 | F | 14.7 | 46.4 | 0.52 | 4.41 | ND | ND | +/+ |
| Median | 11.3 | 28.1 | 0.4 | 2.2 | 0 of 3 | 0 of 4 | 2 of 9 | |
| Myelomonocytic leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 4447 | M | 10.2 | 195 | 1.13 | 3.53 | ND | ND | ND |
| 4448 | M | 8.5 | 7.6 | 0.81 | 1.91 | No CAs | ND | ND |
| 4757 | M | 9.8 | 38 | 0.78 | 3.54 | ND | 41; XY; +X | (+)/(+) |
| 3761 | M | 12.2 | 225 | 1.09 | 5.16 | No CAs | Normal | (+)/(+) |
| 4273 | F | 10.2 | 74 | 0.55 | 1.4 | ND | ND | ND |
| 4122 | F | 11.0 | 480 | 0.57 | 2 | No CAs | Normal | ND |
| Median | 10.2 | 134.5 | 0.8 | 2.8 | 0 of 4 | 1 of 3 | 2 of 2 | |
| Lymphatic leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 3782 | M | 12.3 | 826 | 0.7 | 2.87 | ND | ND | ND |
| 4430 | F | 14.9 | 675 | 0.54 | 3.04 | ND | Gain 9A | +/+ |
| 4115 | F | 10.8 | 76.3 | 0.89 | 3.36 | ND | 42, XX, +14, +17 | (+)/(+) |
| Median | 12.3 | 750.5 | 0.7 | 3.0 | ND | 2 of 2 | 2 of 2 | |
| Biphenotypic leukemia/ Icsbp−/−/Nf1+/− | ||||||||
| 4765 | M | 10.7 | 1148 | 0.67 | 5.57 | ND | ND | (+)/(?) |
| 4453 | F | 11.2 | 568 | 0.87 | 4.29 | ND | 39, X, -X | +/+ |
| Median | 11.8 | 858.0 | 0.8 | 4.9 | ND | 1 of 1 | 2 of 2 | |
| Leukemia without maturation/Icsbp−/−Nf1+/− | ||||||||
| 4289 | F | 13.6 | 773 | 0.51 | 2.73 | ND | Normal | ND |
| Lymphoma/Icsbp−/−Nf1+/− | ||||||||
| 3529 | M | 11.1 | ND | ND | ND | ND | ND | ND |
| MPD-like myeloid leukemia/Icsbp−/−Nf1+/+ | ||||||||
| 3502 | M | 17.4 | 70.1 | 0.32 | 1.74 | ND | ND | ND |
| 4922 | F | 12 | 30.9 | 0.29 | 2.28 | ND | ND | ND |
| Lymphatic leukemia/ Icsbp−/−Nf1+/+ | ||||||||
| 4921 | F | 7.1 | 476 | 1.2 | 4.44 | ND | ND | ND |
| Leukemia without maturation/Icsbp−/−Nf1+/+ | ||||||||
| 4435 | M | 14.2 | 287 | ND | ND | ND | ND | ND |
| Myelomonocytic leukemia/Icsbp+/+Nf1+/− | ||||||||
| 4712 | F | 8.4 | 58.5 | 1.96 | 3.04 | ND | ND | −/− |
Mice were grouped according to genotype and then according to diagnosis. Data show disease characteristics of each individual mouse as well as genetic characteristics of each leukemia. Shown are spectral karyotyping (SKY), microarray-based comparative genomic hybridization (array CGH), and imbalance of allelic expression of Nf1 and LOH of each individual leukemia.
WT indicates wild-type; M, male; F, female; CA, clonal aberration; and ND, not done.