Hematologic parameters in mice with total IRP2 deficiency versus mice lacking IRP2 in enterocytes, hepatocytes, or macrophages
| Genotype . | n . | RBC, 1012/L . | Hematocrit, L/L . | Hemoglobin, g/L . | MCV, fL . |
|---|---|---|---|---|---|
| Males | |||||
| Ireb2+/+ | 8 | 10.9 ± 0.1 | 0.58 ± 0.01 | 161 ± 1 | 52.8 ± 0.2 |
| Ireb2Δ/Δ | 8 | 10.2 ± 0.3 | 0.49 ± 0.01‡ | 138 ± 1‡ | 48.0 ± 0.3‡ |
| Ireb2VilCre(−) | 10 | 11.1 ± 0.2 | 0.58 ± 0.01 | 161 ± 2 | 52.7 ± 0.4 |
| Ireb2VilCre(+) | 9 | 11.3 ± 0.2 | 0.59 ± 0.01 | 169 ± 6 | 53.0 ± 0.3 |
| Ireb2AlfpCre(−) | 8 | 10.9 ± 0.3 | 0.57 ± 0.01 | 157 ± 3 | 52.8 ± 0.3 |
| Ireb2AlfpCre(+) | 8 | 10.8 ± 0.2 | 0.58 ± 0.01 | 161 ± 2 | 54.1 ± 0.3* |
| Ireb2LysMCre(−) | 6 | 10.7 ± 0.2 | 0.53 ± 0.01 | 169 ± 1 | 52.2 ± 0.3 |
| Ireb2LysMCre(+) | 9 | 10.7 ± 0.2 | 0.55 ± 0.01 | 168 ± 1 | 51.9 ± 0.3 |
| Females | |||||
| Ireb2+/+ | 8 | 10.3 ± 0.2 | 0.55 ± 0.01 | 160 ± 1 | 53.5 ± 0.4 |
| Ireb2Δ/Δ | 8 | 9.8 ± 0.1* | 0.48 ± 0.01‡ | 138 ± 1‡ | 49.8 ± 0.2‡ |
| Ireb2VilCre(−) | 10 | 10.9 ± 0.4 | 0.59 ± 0.01 | 166 ± 4 | 54.2 ± 0.8 |
| Ireb2VilCre(+) | 10 | 10.7 ± 0.2 | 0.58 ± 0.01 | 163 ± 3 | 54.3 ± 0.3 |
| Ireb2AlfpCre(−) | 8 | 10.7 ± 0.2 | 0.57 ± 0.01 | 161 ± 1 | 53.3 ± 0.2 |
| Ireb2AlfpCre(+) | 8 | 10.4 ± 0.1 | 0.57 ± 0.01 | 160 ± 1 | 54.6 ± 0.3† |
| Ireb2LysMCre(−) | 7 | 9.8 ± 0.4 | 0.52 ± 0.01 | 172 ± 3 | 52.7 ± 0.8 |
| Ireb2LysMCre(+) | 9 | 10.7 ± 0.3 | 0.56 ± 0.02 | 176 ± 2 | 52.6 ± 0.3 |
| Genotype . | n . | RBC, 1012/L . | Hematocrit, L/L . | Hemoglobin, g/L . | MCV, fL . |
|---|---|---|---|---|---|
| Males | |||||
| Ireb2+/+ | 8 | 10.9 ± 0.1 | 0.58 ± 0.01 | 161 ± 1 | 52.8 ± 0.2 |
| Ireb2Δ/Δ | 8 | 10.2 ± 0.3 | 0.49 ± 0.01‡ | 138 ± 1‡ | 48.0 ± 0.3‡ |
| Ireb2VilCre(−) | 10 | 11.1 ± 0.2 | 0.58 ± 0.01 | 161 ± 2 | 52.7 ± 0.4 |
| Ireb2VilCre(+) | 9 | 11.3 ± 0.2 | 0.59 ± 0.01 | 169 ± 6 | 53.0 ± 0.3 |
| Ireb2AlfpCre(−) | 8 | 10.9 ± 0.3 | 0.57 ± 0.01 | 157 ± 3 | 52.8 ± 0.3 |
| Ireb2AlfpCre(+) | 8 | 10.8 ± 0.2 | 0.58 ± 0.01 | 161 ± 2 | 54.1 ± 0.3* |
| Ireb2LysMCre(−) | 6 | 10.7 ± 0.2 | 0.53 ± 0.01 | 169 ± 1 | 52.2 ± 0.3 |
| Ireb2LysMCre(+) | 9 | 10.7 ± 0.2 | 0.55 ± 0.01 | 168 ± 1 | 51.9 ± 0.3 |
| Females | |||||
| Ireb2+/+ | 8 | 10.3 ± 0.2 | 0.55 ± 0.01 | 160 ± 1 | 53.5 ± 0.4 |
| Ireb2Δ/Δ | 8 | 9.8 ± 0.1* | 0.48 ± 0.01‡ | 138 ± 1‡ | 49.8 ± 0.2‡ |
| Ireb2VilCre(−) | 10 | 10.9 ± 0.4 | 0.59 ± 0.01 | 166 ± 4 | 54.2 ± 0.8 |
| Ireb2VilCre(+) | 10 | 10.7 ± 0.2 | 0.58 ± 0.01 | 163 ± 3 | 54.3 ± 0.3 |
| Ireb2AlfpCre(−) | 8 | 10.7 ± 0.2 | 0.57 ± 0.01 | 161 ± 1 | 53.3 ± 0.2 |
| Ireb2AlfpCre(+) | 8 | 10.4 ± 0.1 | 0.57 ± 0.01 | 160 ± 1 | 54.6 ± 0.3† |
| Ireb2LysMCre(−) | 7 | 9.8 ± 0.4 | 0.52 ± 0.01 | 172 ± 3 | 52.7 ± 0.8 |
| Ireb2LysMCre(+) | 9 | 10.7 ± 0.3 | 0.56 ± 0.02 | 176 ± 2 | 52.6 ± 0.3 |
Hematologic parameters were determined at 10 weeks of age in Ireb2+/+ versus Ireb2Δ/Δ mice with total and constitutive IRP2 deficiency and in Ireb2VilCre, Ireb2AlfpCre, and Ireb2LysMCre mice with (+) or without (−) selective IRP2 ablation in intestinal epithelial cells, in hepatocytes, or in macrophages, respectively. Data are given as average plus or minus SEM. The sample size (n) is indicated.
RBC indicates red blood cell; MCV, mean corpuscular volume; and WBC, white blood cell.
Data were compared between control and IRP2-deficient animals within each group:
P < .05;
P < .01;
P < .001, Student t test.