Ex vivo frequency of CD8+ T cells lacking the costimulatory molecules in peripheral blood mononuclear cells from HTLV-1–infected patients and uninfected healthy controls
| . | n . | Mean ± SE . | P (ANOVA) . | P (Sheffe F) . | |
|---|---|---|---|---|---|
| HAM-HCs . | HVCs-HCs . | ||||
| All (PVL-unmatched) | |||||
| PVL | |||||
| HAM/TSP | 16 | 805.1 ± 461.0 | .001 | N/A | N/A |
| HVCs | 20 | 354.5 ± 106.2 | (Mann-Whitney) | ||
| %CD8+CD27−* | |||||
| HAM/TSP | 16 | 31.6 ± 2.3 | |||
| HVCs | 20 | 22.3 ± 1.7 | .003 | .004 | .83 |
| HCs | 20 | 19.7 ± 1.3 | |||
| %CD8+CD28− | |||||
| HAM/TSP | 16 | 31.4 ± 3.1 | |||
| HVCs | 20 | 23.6 ± 1.5 | .003 | .003 | .91 |
| HCs | 20 | 20.3 ± 1.5 | |||
| %CD8+CD80− | |||||
| HAM/TSP | 16 | 36.4 ± 3.3 | |||
| HVCs | 20 | 32.3 ± 1.6 | .015 | .012 | .20 |
| HCs | 20 | 26.2 ± 1.7 | |||
| %CD8+CD86− | |||||
| HAM/TSP | 16 | 35.3 ± 3.2 | |||
| HVCs | 20 | 30.6 ± 1.5 | .009 | .007 | .23 |
| HCs | 20 | 25.1 ± 1.5 | |||
| %CD8+CD152− | |||||
| HAM/TSP | 16 | 35.6 ± 2.5 | |||
| HVCs | 20 | 30.1 ± 1.9 | .004 | .003 | .28 |
| HCs | 20 | 25.2 ± 1.7 | |||
| PVL-matched | |||||
| PVL | |||||
| HAM/TSP | 8 | 529.8 ± 79.0 | .79 | N/A | N/A |
| HVCs | 9 | 486.0 ± 104.5 | (Mann-Whitney) | ||
| %CD8+CD27− | |||||
| HAM/TSP | 8 | 31.8 ± 5.9 | |||
| HVCs | 9 | 19.8 ± 3.6 | .012 | .009 | 1.0 |
| HCs | 20 | 19.7 ± 1.3 | |||
| %CD8+CD28− | |||||
| HAM/TSP | 8 | 30.6 ± 3.2 | |||
| HVCs | 9 | 22.6 ± 2.4 | .006 | .007 | .78 |
| HCs | 20 | 20.3 ± 1.5 | |||
| %CD8+CD80− | |||||
| HAM/TSP | 8 | 39.5 ± 3.8 | |||
| HVCs | 9 | 32.5 ± 2.1 | .001 | .001 | .16 |
| HCs | 20 | 26.2 ± 1.7 | |||
| %CD8+CD86− | |||||
| HAM/TSP | 8 | 35.3 ± 4.2 | |||
| HVCs | 9 | 31.8 ± 1.8 | .007 | .013 | .11 |
| HCs | 20 | 25.1 ± 1.5 | |||
| %CD8+CD152− | |||||
| HAM/TSP | 8 | 31.0 ± 4.2 | |||
| HVCs | 9 | 30.5 ± 2.8 | .19 | .32 | .35 |
| HCs | 20 | 25.2 ± 1.7 | |||
| . | n . | Mean ± SE . | P (ANOVA) . | P (Sheffe F) . | |
|---|---|---|---|---|---|
| HAM-HCs . | HVCs-HCs . | ||||
| All (PVL-unmatched) | |||||
| PVL | |||||
| HAM/TSP | 16 | 805.1 ± 461.0 | .001 | N/A | N/A |
| HVCs | 20 | 354.5 ± 106.2 | (Mann-Whitney) | ||
| %CD8+CD27−* | |||||
| HAM/TSP | 16 | 31.6 ± 2.3 | |||
| HVCs | 20 | 22.3 ± 1.7 | .003 | .004 | .83 |
| HCs | 20 | 19.7 ± 1.3 | |||
| %CD8+CD28− | |||||
| HAM/TSP | 16 | 31.4 ± 3.1 | |||
| HVCs | 20 | 23.6 ± 1.5 | .003 | .003 | .91 |
| HCs | 20 | 20.3 ± 1.5 | |||
| %CD8+CD80− | |||||
| HAM/TSP | 16 | 36.4 ± 3.3 | |||
| HVCs | 20 | 32.3 ± 1.6 | .015 | .012 | .20 |
| HCs | 20 | 26.2 ± 1.7 | |||
| %CD8+CD86− | |||||
| HAM/TSP | 16 | 35.3 ± 3.2 | |||
| HVCs | 20 | 30.6 ± 1.5 | .009 | .007 | .23 |
| HCs | 20 | 25.1 ± 1.5 | |||
| %CD8+CD152− | |||||
| HAM/TSP | 16 | 35.6 ± 2.5 | |||
| HVCs | 20 | 30.1 ± 1.9 | .004 | .003 | .28 |
| HCs | 20 | 25.2 ± 1.7 | |||
| PVL-matched | |||||
| PVL | |||||
| HAM/TSP | 8 | 529.8 ± 79.0 | .79 | N/A | N/A |
| HVCs | 9 | 486.0 ± 104.5 | (Mann-Whitney) | ||
| %CD8+CD27− | |||||
| HAM/TSP | 8 | 31.8 ± 5.9 | |||
| HVCs | 9 | 19.8 ± 3.6 | .012 | .009 | 1.0 |
| HCs | 20 | 19.7 ± 1.3 | |||
| %CD8+CD28− | |||||
| HAM/TSP | 8 | 30.6 ± 3.2 | |||
| HVCs | 9 | 22.6 ± 2.4 | .006 | .007 | .78 |
| HCs | 20 | 20.3 ± 1.5 | |||
| %CD8+CD80− | |||||
| HAM/TSP | 8 | 39.5 ± 3.8 | |||
| HVCs | 9 | 32.5 ± 2.1 | .001 | .001 | .16 |
| HCs | 20 | 26.2 ± 1.7 | |||
| %CD8+CD86− | |||||
| HAM/TSP | 8 | 35.3 ± 4.2 | |||
| HVCs | 9 | 31.8 ± 1.8 | .007 | .013 | .11 |
| HCs | 20 | 25.1 ± 1.5 | |||
| %CD8+CD152− | |||||
| HAM/TSP | 8 | 31.0 ± 4.2 | |||
| HVCs | 9 | 30.5 ± 2.8 | .19 | .32 | .35 |
| HCs | 20 | 25.2 ± 1.7 | |||
HVCs indicates HTLV-1 carriers; HCs, uninfected healthy controls; and PVL, proviral load.
One-factor ANOVA was done when variance of each group was equal by Levene test. If variance of each group was different, Kruskal-Wallis test was used. For multiple comparison, we used Sheffe F to analyze the statistical difference.
% CD8+CD27− indicates the frequency of CD8+CD27− in PBMCs.