Properties of the CD30-specific antibody and huTR formats
| Antibody and huTR formats . | Binding to CD30 (ELISA) . | Apparent KD[M] . | Binding to CD30+ cells (FACS) . | In vitro cytotoxicity . |
|---|---|---|---|---|
| scFv | +++ | 5.66 − 10−7* | +/− | ND |
| scFv-RNase | +++ | 4.32 − 10−7* | ND | ND |
| IgG | +++ | 1.68 − 10−8 | +/− | ND |
| scFv-RNase-Fc | +++ | 7.43 − 10−10 | +++ | ND |
| scFv-Fc | +++ | 6.70 − 10−9 | +++ | − |
| scFv-Fc-RNase | +++ | 9.81 − 10−10 | +++ | +++ |
| Antibody and huTR formats . | Binding to CD30 (ELISA) . | Apparent KD[M] . | Binding to CD30+ cells (FACS) . | In vitro cytotoxicity . |
|---|---|---|---|---|
| scFv | +++ | 5.66 − 10−7* | +/− | ND |
| scFv-RNase | +++ | 4.32 − 10−7* | ND | ND |
| IgG | +++ | 1.68 − 10−8 | +/− | ND |
| scFv-RNase-Fc | +++ | 7.43 − 10−10 | +++ | ND |
| scFv-Fc | +++ | 6.70 − 10−9 | +++ | − |
| scFv-Fc-RNase | +++ | 9.81 − 10−10 | +++ | +++ |
ELISA indicates enzyme-linked immunosorbent assay; FACS, fluorescence-activated cell sorter; and ND, not determined.
Steady-state analysis.