Percentages of erythroid progenitor populations in the bone marrow and spleen of humanized CA and control mice by flow cytometry
| Tissue . | Cells . | Region . | Percentage of total cells (%) . | P . | |
|---|---|---|---|---|---|
| Control mice . | CA mice . | ||||
| Bone marrow | Proerythroblasts | I | 0.13 (± 0.03) | 0.82 (± 0.21) | .0188 |
| Early erythroblasts | II | 27.9 (± 5.6) | 56.0 (± 3.1) | .0046 | |
| Late erythroblasts | III | 11.9 (± 2.9) | 4.0 (± 0.8) | .04 | |
| Total Ter119+ | II + III | 39.7 (± 4.2) | 60.1 (± 2.6) | .0059 | |
| Spleen | Proerythroblasts | I | 0.10 (± 0.04) | 0.70 (± 0.16) | .0126 |
| Early erythroblasts | II | 15.6 (± 6.7) | 74.9 (± 5.6) | .0005 | |
| Late erythroblasts | III | 34.1 (± 3.6) | 8.3 (± 1.9) | .0007 | |
| Total Ter119+ | II + III | 49.7 (± 3.4) | 83.2 (± 6.9) | .0048 | |
| Tissue . | Cells . | Region . | Percentage of total cells (%) . | P . | |
|---|---|---|---|---|---|
| Control mice . | CA mice . | ||||
| Bone marrow | Proerythroblasts | I | 0.13 (± 0.03) | 0.82 (± 0.21) | .0188 |
| Early erythroblasts | II | 27.9 (± 5.6) | 56.0 (± 3.1) | .0046 | |
| Late erythroblasts | III | 11.9 (± 2.9) | 4.0 (± 0.8) | .04 | |
| Total Ter119+ | II + III | 39.7 (± 4.2) | 60.1 (± 2.6) | .0059 | |
| Spleen | Proerythroblasts | I | 0.10 (± 0.04) | 0.70 (± 0.16) | .0126 |
| Early erythroblasts | II | 15.6 (± 6.7) | 74.9 (± 5.6) | .0005 | |
| Late erythroblasts | III | 34.1 (± 3.6) | 8.3 (± 1.9) | .0007 | |
| Total Ter119+ | II + III | 49.7 (± 3.4) | 83.2 (± 6.9) | .0048 | |
Statistical significances were determined for the CA mice compared with control mice. Erythroid populations of humanized HbA mice (α2α1/α2α1 γβA/γβA) and C57BL/6J control mice do not differ significantly (data not shown). Percentages are mean (± SEM). n = 4 in each group. P values were calculated by 2-tailed unpaired Student t test.