N-linked glycosylation profile of 2F8 glycovariants
| . | 2F8-H . | 2F8-HEK . | 2F8-C . | 2F8-HEK−(F+/G−/S−) . | 2F8-C−(F−/G−/S−) . |
|---|---|---|---|---|---|
| Origin | Hybridoma | HEK-293F | CHO-DG44 | HEK-293F | CHO-DG44 |
| Treatment | Mock/none | Mock | Mock/none | Galactosidase; neuramidase | Galactosidase; neuramidase |
| Fucose, % | 95.1 | 95.1 | 75.9 | 95.3 | 75.9 |
| Galactose, % | 73.8 | 33.1 | 26.1 | 3.3 | 0.2 |
| Sialic acids, % | 55.3 | 1.8 | 3.0 | 0.7 | 2.0 |
| . | 2F8-H . | 2F8-HEK . | 2F8-C . | 2F8-HEK−(F+/G−/S−) . | 2F8-C−(F−/G−/S−) . |
|---|---|---|---|---|---|
| Origin | Hybridoma | HEK-293F | CHO-DG44 | HEK-293F | CHO-DG44 |
| Treatment | Mock/none | Mock | Mock/none | Galactosidase; neuramidase | Galactosidase; neuramidase |
| Fucose, % | 95.1 | 95.1 | 75.9 | 95.3 | 75.9 |
| Galactose, % | 73.8 | 33.1 | 26.1 | 3.3 | 0.2 |
| Sialic acids, % | 55.3 | 1.8 | 3.0 | 0.7 | 2.0 |
N-linked glycans of 2F8 were enzymatically released and analyzed by HPAEC-PAD. Percentages were calculated using the relative peak area in the elution profiles.