DC progenitor populations
| Group/precursor population name . | Site of isolation . | Ex vivo sorting immunophenotype . | Frequency, percentage . | Differentiation potential in vitro . | Clonal potential in vitro . | Differentiation potential in vivo . | Conditions . | Peak day of progeny and fold expansion of input in spleen . | References . |
|---|---|---|---|---|---|---|---|---|---|
| Early DC progenitors (c-Kit+, high proliferation potential) | |||||||||
| MDPs (macrophage and DC progenitor) | BM | Lin−CD117+CX3CR1+CD11b− | 0.5 | DC, Mac | DC, Mac | S, peritoneum: Mo, DC, Mac | U, RT, IF peritoneum | Day 7, 10× (RT) | 89 |
| MDPD (macrophage and DC progenitor) | BM | Lin−CD117+/−CD115+ | 0.2 | NA | NA | S:DC, Mac | RT | NA | 90 |
| CDPs (common DC progenitors) | BM | Lin−CD117intCD135+ CD115+ CD127− | 0.1 | pDC, DC | pDC, DC | S, LN: pDC, DC | U, RT | Day 10, 7-8× (RT) | 58 |
| Pro-DCs | BM | Lin−CD117intCD135+ CD16/32lo | NA | pDC, DC | pDC, DC | S: pDC, DC | U | Day 8?, 0.05×* | 91 |
| Late DC progenitors (c-Kit−, low proliferation potential) | |||||||||
| DC progenitors B220+ | BM | CD11c+MHC class II−B220+ | 0.3 | pDC, DC | NA | S: pDC, DC | RT | Day 7?, 0.01× (RT) | 92 |
| DC progenitors B220− | BM | CD11c+MHC class II−B220− | 0.2 | DC | NA | S: DC | RT | Day 7?, 0.03× (RT) | 92 |
| Preimmunocyte | BM | CD11c+CD31+Ly6C+ | 0.5-1 | pDC, DC, Mac | NA | NA | NA | NA | 93 |
| DC precursors | Blood | CD11c+MHC class II− | 5 | NA | NA | S: pDC, DC | RT | Day 14, 1× | 36 † |
| Pre-cDCs | Spleen | CD11cintCD45RAlo CD43intSIRP-aint | 0.05 | DC | NA | S: DC | U | Day 5, 0.02×* | 94 |
| Monocytes (no proliferation potential) | |||||||||
| Monocytes | BM | Ly6Chi (Gr-1hi) | NA | NA | DC | IF spleen | Day 2 | 94 | |
| Monocytes | Blood | Gr-1hi | NA | NA | LC | IF skin | 56 | ||
| Monocytes | BM | Gr-1hi inflammatory | NA | NA | DC | IF intestine + lung | 97 | ||
| Monocytes | BM | Gr-1hi and Gr-1− | NA | NA | DC | IF lung | 96 | ||
| Monocytes | Blood | Gr-1hi | NA | NA | DC | IF vagina | 12 | ||
| Group/precursor population name . | Site of isolation . | Ex vivo sorting immunophenotype . | Frequency, percentage . | Differentiation potential in vitro . | Clonal potential in vitro . | Differentiation potential in vivo . | Conditions . | Peak day of progeny and fold expansion of input in spleen . | References . |
|---|---|---|---|---|---|---|---|---|---|
| Early DC progenitors (c-Kit+, high proliferation potential) | |||||||||
| MDPs (macrophage and DC progenitor) | BM | Lin−CD117+CX3CR1+CD11b− | 0.5 | DC, Mac | DC, Mac | S, peritoneum: Mo, DC, Mac | U, RT, IF peritoneum | Day 7, 10× (RT) | 89 |
| MDPD (macrophage and DC progenitor) | BM | Lin−CD117+/−CD115+ | 0.2 | NA | NA | S:DC, Mac | RT | NA | 90 |
| CDPs (common DC progenitors) | BM | Lin−CD117intCD135+ CD115+ CD127− | 0.1 | pDC, DC | pDC, DC | S, LN: pDC, DC | U, RT | Day 10, 7-8× (RT) | 58 |
| Pro-DCs | BM | Lin−CD117intCD135+ CD16/32lo | NA | pDC, DC | pDC, DC | S: pDC, DC | U | Day 8?, 0.05×* | 91 |
| Late DC progenitors (c-Kit−, low proliferation potential) | |||||||||
| DC progenitors B220+ | BM | CD11c+MHC class II−B220+ | 0.3 | pDC, DC | NA | S: pDC, DC | RT | Day 7?, 0.01× (RT) | 92 |
| DC progenitors B220− | BM | CD11c+MHC class II−B220− | 0.2 | DC | NA | S: DC | RT | Day 7?, 0.03× (RT) | 92 |
| Preimmunocyte | BM | CD11c+CD31+Ly6C+ | 0.5-1 | pDC, DC, Mac | NA | NA | NA | NA | 93 |
| DC precursors | Blood | CD11c+MHC class II− | 5 | NA | NA | S: pDC, DC | RT | Day 14, 1× | 36 † |
| Pre-cDCs | Spleen | CD11cintCD45RAlo CD43intSIRP-aint | 0.05 | DC | NA | S: DC | U | Day 5, 0.02×* | 94 |
| Monocytes (no proliferation potential) | |||||||||
| Monocytes | BM | Ly6Chi (Gr-1hi) | NA | NA | DC | IF spleen | Day 2 | 94 | |
| Monocytes | Blood | Gr-1hi | NA | NA | LC | IF skin | 56 | ||
| Monocytes | BM | Gr-1hi inflammatory | NA | NA | DC | IF intestine + lung | 97 | ||
| Monocytes | BM | Gr-1hi and Gr-1− | NA | NA | DC | IF lung | 96 | ||
| Monocytes | Blood | Gr-1hi | NA | NA | DC | IF vagina | 12 | ||
Data acquired in cellular transfer models.
U indicates unconditioned animals (ie, “steady-state” differentiation upon transfer in nonirradiated and noninflamed animals); RT, irradiation before transfer; IF, induction of inflammation before or after transfer; S, spleen; LC, Langerhans cell; and NA, not available.
Expansion in nonirradiated animals cannot be compared with expansion in irradiated animals.
Population contained DX5+ NK cells; purification of restricted blood DC precursors remains to be established.