Pathologic GVHD
. | WT Allo-HCT . | GKO Allo-HCT . | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Spc + BMC . | Spc . | Spc + BMC . | Spc . | ||||||||
DOD | 16 | 48 | 13 | 14 | 14 | 21 | 21 | 26 | 17 | 17 | 17 |
Liver | − | + | + | + | ++ | + | + | ++ | ++ | +++ | + |
Lung | − | − | − | − | − | + | ++ | +++ | +++ | +++ | ++ |
. | WT Allo-HCT . | GKO Allo-HCT . | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Spc + BMC . | Spc . | Spc + BMC . | Spc . | ||||||||
DOD | 16 | 48 | 13 | 14 | 14 | 21 | 21 | 26 | 17 | 17 | 17 |
Liver | − | + | + | + | ++ | + | + | ++ | ++ | +++ | + |
Lung | − | − | − | − | − | + | ++ | +++ | +++ | +++ | ++ |
Recipient mice received 6 Gy TBI followed by administration of 3 × 107 splenocytes alone (Spc) or along with 107 bone marrow cells (Spc + BMC) from WT or GKO B6 donors. Carcasses were saved in 10% formalin after death. Tissue sections were examined blindly by a pathologist and graded as follows: − indicates no detectable lesions; +, minimal lesions; ++, moderate lesions; +++, severe lesions.
DOD indicates date of death (days after HCT).