Fall-off of hFIX in synovial fluid after intraarticular administration
| Route/tissue . | 1 hours . | 4 hours . | 12 hours . | 24 hours . | 48 hours . | 72 hours . |
|---|---|---|---|---|---|---|
| Intraarticular SF | 11 511 ± 1155 | 5509 ± 896 | 384 ± 81.8 | 116 ± 32.0 | 52.3 ± 10.0 | 26.1 ± 3.6 |
| Intravenous SF | 12.5 ± 2.1 | 6.42 ± 2.88 | ND | ND | ND | ND |
| Intravenous plasma | 2530 ± 268 | 1552 ± 138 | 584 ± 18.5 | 123 ± 24.2 | 52.2 ± 5.9 | 24.3 ± 1.8 |
| Route/tissue . | 1 hours . | 4 hours . | 12 hours . | 24 hours . | 48 hours . | 72 hours . |
|---|---|---|---|---|---|---|
| Intraarticular SF | 11 511 ± 1155 | 5509 ± 896 | 384 ± 81.8 | 116 ± 32.0 | 52.3 ± 10.0 | 26.1 ± 3.6 |
| Intravenous SF | 12.5 ± 2.1 | 6.42 ± 2.88 | ND | ND | ND | ND |
| Intravenous plasma | 2530 ± 268 | 1552 ± 138 | 584 ± 18.5 | 123 ± 24.2 | 52.2 ± 5.9 | 24.3 ± 1.8 |
FIX−/− mice were given recombinant hFIX by intravenous or intraarticular administration at the dose of 100 IU/kg, n ≥ 6 at each time point. All samples are detected via hFIX specific ELISA, reported as ng FIX/mL. The normal circulating level of FIX protein in human plasma is 5000 ng/mL.
SF indicates synovial fluid; IA, intraarticular FIX delivery; IV, intravenous FIX delivery by tail vein; and ND, not detectable.