Factor IX survival in circulation
| Mouse . | Dose/route . | 15 minutes . | 1 hour . | 2 hours . |
|---|---|---|---|---|
| Initial circulating FIX activity in mouse plasma after recombinant hFIX | ||||
| FIX−/− | 20 IU/kg IA | < 1% | < 1% | < 1% |
| FIX−/− | 20 IU/kg IV | 1.95 ± 0.57 | 1.63 ± 0.68 | < 1% |
| FIX−/− | 80 IU/kg IV | 15.7 ± 5.88 | 11.5 ± 5.56 | 8.75 ± 3.46 |
| Mouse . | Dose/route . | 15 minutes . | 1 hour . | 2 hours . |
|---|---|---|---|---|
| Initial circulating FIX activity in mouse plasma after recombinant hFIX | ||||
| FIX−/− | 20 IU/kg IA | < 1% | < 1% | < 1% |
| FIX−/− | 20 IU/kg IV | 1.95 ± 0.57 | 1.63 ± 0.68 | < 1% |
| FIX−/− | 80 IU/kg IV | 15.7 ± 5.88 | 11.5 ± 5.56 | 8.75 ± 3.46 |
| Mouse . | Dose/route . | 1 hour . | 4 hours . | 12 hours . | 24 hours . | 48 hours . | 72 hours . |
|---|---|---|---|---|---|---|---|
| Extended circulating FIX activity in mouse plasma after recombinant hFIX | |||||||
| FIX −/− (n = 6) | 25 IU/kg IA | < 1% | < 1% | < 1% | < 1% | < 1% | < 1% |
| FIX −/− (n = 7) | 100 IU/kg IA | < 1% | < 1% | < 1% | < 1% | < 1% | < 1% |
| FIX −/− (n = 8) | 25 IU/kg IV | 4.5 ± 0.9 | 2.1 ± 0.6 | 1.4 ± 0.4 | 1.0 ± 0.6 | < 1% | < 1% |
| FIX −/− (n = 9) | 100 IU/kg IV | 17.3 ± 3.3 | 11.8 ± 4.8 | 6.4 ± 2.1 | 3.8 ± 1.8 | 2.7 ± 1.1 | 1.5 ± 0.4 |
| Mouse . | Dose/route . | 1 hour . | 4 hours . | 12 hours . | 24 hours . | 48 hours . | 72 hours . |
|---|---|---|---|---|---|---|---|
| Extended circulating FIX activity in mouse plasma after recombinant hFIX | |||||||
| FIX −/− (n = 6) | 25 IU/kg IA | < 1% | < 1% | < 1% | < 1% | < 1% | < 1% |
| FIX −/− (n = 7) | 100 IU/kg IA | < 1% | < 1% | < 1% | < 1% | < 1% | < 1% |
| FIX −/− (n = 8) | 25 IU/kg IV | 4.5 ± 0.9 | 2.1 ± 0.6 | 1.4 ± 0.4 | 1.0 ± 0.6 | < 1% | < 1% |
| FIX −/− (n = 9) | 100 IU/kg IV | 17.3 ± 3.3 | 11.8 ± 4.8 | 6.4 ± 2.1 | 3.8 ± 1.8 | 2.7 ± 1.1 | 1.5 ± 0.4 |
FIX−/− mice were given recombinant hFIX by intravenous or intraarticular administration at the dose of 20 IU/kg or 80 IU/kg for initial factor IX activity recovery or 25 IU/kg or 100 IU/kg for extended activity recovery. Citrated plasma was collected for FIX activity assay (aPTT), and data are presented as percentage of normal human plasma activity.
IA indicates intraarticular; and IV, intravenous by tail vein.