Table 1 Markers used to define HPC and... | American Society of Hematology

Table 1

Markers used to define HPC and lineage cells

Hematopoietic lineage cells	Marker
T-cell lineage
THY
DN1	CD25⁻ CD44⁺ Lin⁻
DN2	CD25⁺ CD44⁺ Lin⁻
DN3	CD25⁺ CD44⁻ Lin⁻
DN4	CD25⁻ CD44⁻ Lin⁻
DP	CD4⁺ CD8⁺
THY/SPL
CD4 SP	CD4⁺ TCR^hi
CD8 SP	CD8⁺ TCR^hi
nTreg	Foxp3⁺ CD4⁺ CD25⁺
NKT	TCR⁺ CD1d⁺
B-cell lineage
BM
HPC	Sca1⁺ ckit⁺ Lin⁻*
Fr A	CD43⁺ B220^lo CD24⁻
Fr B+C	CD43⁺ B220^lo CD24⁺
Fr D	CD43⁻ B220⁺ IgM⁻ IgD⁻ CD93+
Fr E	CD43⁻ B220⁺ IgM⁺ IgD⁻ CD93+
Fr F	CD43⁻ B220⁺ IgM⁺ IgD⁺ CD93−
SPL
T1	CD23⁻ IgM⁺ CD24⁺ CD21^−/lo
T2-FO	CD23⁺ CD24⁺ CD21^lo
FO	CD23⁺ IgM^int CD24^lo CD21⁺
T2-MZP	CD23⁺ IgM^hi CD24⁺ CD21^hi
MZ	CD23⁻ IgM⁺ CD24⁺ CD21^hi
PT
B1a	CD19^hi CD5⁺ B220^lo
B2	CD19^lo CD5⁻ B220^hi
PPs
PNA−	B220⁺ CD19⁺ PNA⁺
PNA+	B220⁺ CD19⁺ PNA⁻
Myeloid cells
BM/SPL
Imm N	Gr1^lo CD11b⁺
Mat N	Gr1^hi CD11b⁺
SPL/culture
NK	Dx5⁺ TCR⁻
DC	CD11b⁺ CD11c⁺
Mo/Ma	CD11b⁺ CD11c⁻
PB
Platelets	CD41⁺ CD61⁺

Hematopoietic lineage cells	Marker
T-cell lineage
THY
DN1	CD25⁻ CD44⁺ Lin⁻
DN2	CD25⁺ CD44⁺ Lin⁻
DN3	CD25⁺ CD44⁻ Lin⁻
DN4	CD25⁻ CD44⁻ Lin⁻
DP	CD4⁺ CD8⁺
THY/SPL
CD4 SP	CD4⁺ TCR^hi
CD8 SP	CD8⁺ TCR^hi
nTreg	Foxp3⁺ CD4⁺ CD25⁺
NKT	TCR⁺ CD1d⁺
B-cell lineage
BM
HPC	Sca1⁺ ckit⁺ Lin⁻*
Fr A	CD43⁺ B220^lo CD24⁻
Fr B+C	CD43⁺ B220^lo CD24⁺
Fr D	CD43⁻ B220⁺ IgM⁻ IgD⁻ CD93+
Fr E	CD43⁻ B220⁺ IgM⁺ IgD⁻ CD93+
Fr F	CD43⁻ B220⁺ IgM⁺ IgD⁺ CD93−
SPL
T1	CD23⁻ IgM⁺ CD24⁺ CD21^−/lo
T2-FO	CD23⁺ CD24⁺ CD21^lo
FO	CD23⁺ IgM^int CD24^lo CD21⁺
T2-MZP	CD23⁺ IgM^hi CD24⁺ CD21^hi
MZ	CD23⁻ IgM⁺ CD24⁺ CD21^hi
PT
B1a	CD19^hi CD5⁺ B220^lo
B2	CD19^lo CD5⁻ B220^hi
PPs
PNA−	B220⁺ CD19⁺ PNA⁺
PNA+	B220⁺ CD19⁺ PNA⁻
Myeloid cells
BM/SPL
Imm N	Gr1^lo CD11b⁺
Mat N	Gr1^hi CD11b⁺
SPL/culture
NK	Dx5⁺ TCR⁻
DC	CD11b⁺ CD11c⁺
Mo/Ma	CD11b⁺ CD11c⁻
PB
Platelets	CD41⁺ CD61⁺

B indicates B cell; B1/B2, peritoneal B cells; BM, bone marrow; culture, bone marrow cells cultured for 7 days with M-CSF or GM-CSF; DC, dendritic cell; DN, CD4CD8 double-negative; DP, CD4CD8 double-positive; FO, follicular; Fr, fraction; HPC, hematopoietic progenitor cells; Mo/Ma, monocyte/macrophage; MZ, marginal zone; N, neutrophil; NK, natural killer cell; NKT, NK T cell; nTregs, naturally occurring T cells; PB, peripheral blood; PNA, peanut agglutinin; PPs, Peyer's patches; PT, peritoneum; SP, CD4 or CD8 single-positive; SPL, spleen; T1, transitional 1 B cell; T2 transitional 2 B cell; T2-FO, transitional 2 follicular precursor B cell; T2-MZP, transitional 2 marginal zone precursor B cell; and THY, thymus.

Lin⁻: CD11b, CD11c, Gr-1, CD4, CD8, B220.

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