Table 2 Summary of culture conditions... | American Society of Hematology

Table 2

Summary of culture conditions and SRC expansion based on data presented in Figures 1 through 5

Figure no.	CB cells	Cytokines added and culture duration	O₂	Average fold increase in nucleated cells	SRC expansion
1	Total nucleated cells	SCF+TPO+FGF-1+IGF-2+Angptl5 19d	Normal	19	+
2	CD34⁺ (fresh)	SCF+TPO+Flt3L 11d	Normal	257	−
2	CD34⁺ (fresh)	SCF+TPO+Flt3L+Angptl5 11d	Normal	283	+
3	CD133⁺	SCF+TPO+Flt3L 10d	Normal	210	−
		SCF+TPO+Flt3L+Angptl5 10d	Normal	220	++
		SCF+TPO+Flt3L+IGFBP2 10d	Normal	162	++
4	CD133⁺	SCF+TPO+FGF-1 11d	Low	203	−
4	CD133⁺	SCF+TPO+FGF-1+Angptl5+IGFBP2 11d	Low	266	++
5	CD133⁺	SCF+TPO+FGF-1+Angptl5+IGFBP2 10d	Normal	231	++
5	CD133⁺	SCF+TPO+FGF-1+Angptl5+IGFBP2 10d	Low	258	++

Figure no.	CB cells	Cytokines added and culture duration	O₂	Average fold increase in nucleated cells	SRC expansion
1	Total nucleated cells	SCF+TPO+FGF-1+IGF-2+Angptl5 19d	Normal	19	+
2	CD34⁺ (fresh)	SCF+TPO+Flt3L 11d	Normal	257	−
2	CD34⁺ (fresh)	SCF+TPO+Flt3L+Angptl5 11d	Normal	283	+
3	CD133⁺	SCF+TPO+Flt3L 10d	Normal	210	−
		SCF+TPO+Flt3L+Angptl5 10d	Normal	220	++
		SCF+TPO+Flt3L+IGFBP2 10d	Normal	162	++
4	CD133⁺	SCF+TPO+FGF-1 11d	Low	203	−
4	CD133⁺	SCF+TPO+FGF-1+Angptl5+IGFBP2 11d	Low	266	++
5	CD133⁺	SCF+TPO+FGF-1+Angptl5+IGFBP2 10d	Normal	231	++
5	CD133⁺	SCF+TPO+FGF-1+Angptl5+IGFBP2 10d	Low	258	++

The symbols denote relative, not absolute, values. ++ represents a greater expansion than +. We used freshly isolated CD34⁺ cells in the experiment depicted in Figure 2, and used cryopreserved cells in other experiments.

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