Table 2 Primers used for quantitative... | American Society of Hematology

Table 2

Primers used for quantitative RT-PCR

DHFR forward (exon 4)

ATGCCTTAAAACTTACTGAACAACCA

DHFR reverse (exon 5)

TGGGTGATTCATGGCTTCCT

β₂-microglobulin forward (exon 1)

TACTCTCTCTTTCTGGCCTG

β₂-microglobulin reverse (exon 2)

GGATGGATGAAACCCAGACA

Primers are designed based on DHFR and β₂-microglobulin mRNA sequences (Genbank accession nos. NM_000791 and NM_004048, respectively). The sequence of DHFR primers are as in Serra et al.⁵

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