Table 2 Knockdown of HPRT and treatment... | American Society of Hematology

Table 2

Knockdown of HPRT and treatment with 6TG allows for selection of primitive hematopoietic cells in secondary recipients

Donor treatmentby animal ID	Mean GFP%, 4 wk*			Mean GFP%, 8 wk (n)
Donor treatmentby animal ID	WBC	Gr1	B220	WBC	Gr1	B220
Background	0.08%	0.02%	0.03%	0.05%	0.02%	0.02%
PBS
988	0.09%	0.01%	0.06%	0.07% (3)	0.04% (3)	0.02% (3)
989	0.09%	0.09%	0.05%	0.08% (3)	0.05% (2)	0.04% (2)
992
993	0.12%	0.01%	0.23%	0.08% (2)	0.05% (2)	0.03% (2)
995	0.08%	0.09%	0.02%	0.06% (2)	0.03 (2)	0.02% (2)
Mean	0.10%	0.05%	0.09%	0.07%	0.04%	0.03%
6TG
990	0.90%	0.08%	3.17%	0.14% (1)	0.02% (1)	0.49% (1)
991	7.03%	12.28%	3.25%	0.41% (3)	1.15% (3)	0.39% (3)
994	0.29%	0.11%	0.58%	0.08% (2)	0.03% (2)	0.09% (2)
996
997	10.20%	13.93%	1.86%	0.77% (3)	2.49% (3)	2.61% (3)
Mean	4.61%	6.60%	2.22%	0.35%	0.92%	0.90%

Donor treatmentby animal ID	Mean GFP%, 4 wk*			Mean GFP%, 8 wk (n)
Donor treatmentby animal ID	WBC	Gr1	B220	WBC	Gr1	B220
Background	0.08%	0.02%	0.03%	0.05%	0.02%	0.02%
PBS
988	0.09%	0.01%	0.06%	0.07% (3)	0.04% (3)	0.02% (3)
989	0.09%	0.09%	0.05%	0.08% (3)	0.05% (2)	0.04% (2)
992
993	0.12%	0.01%	0.23%	0.08% (2)	0.05% (2)	0.03% (2)
995	0.08%	0.09%	0.02%	0.06% (2)	0.03 (2)	0.02% (2)
Mean	0.10%	0.05%	0.09%	0.07%	0.04%	0.03%
6TG
990	0.90%	0.08%	3.17%	0.14% (1)	0.02% (1)	0.49% (1)
991	7.03%	12.28%	3.25%	0.41% (3)	1.15% (3)	0.39% (3)
994	0.29%	0.11%	0.58%	0.08% (2)	0.03% (2)	0.09% (2)
996
997	10.20%	13.93%	1.86%	0.77% (3)	2.49% (3)	2.61% (3)
Mean	4.61%	6.60%	2.22%	0.35%	0.92%	0.90%

Flow cytometric data from peripheral blood of secondary recipients of BM from PBS- and 6TG-treated donors.

n = 3 secondary recipients per group.