The effect of IRF8 expression on cell growth
| . | Fold increase of cell numbers . | ||
|---|---|---|---|
| eGFP . | eGFP-Irf8 . | eGFP-K79E . | |
| Expt 1* | |||
| IRF8+/+ HSCs | 50.6 | 30.9 | 47.2 |
| IRF8−/− HSCs | 190.3 | 69.8 | 174.3 |
| Expt 2† | |||
| IRF8+/+ HSCs | 21.3 | 10.3 | 26.0 |
| IRF8−/− HSCs | 35.2 | 15.8 | 35.2 |
| . | Fold increase of cell numbers . | ||
|---|---|---|---|
| eGFP . | eGFP-Irf8 . | eGFP-K79E . | |
| Expt 1* | |||
| IRF8+/+ HSCs | 50.6 | 30.9 | 47.2 |
| IRF8−/− HSCs | 190.3 | 69.8 | 174.3 |
| Expt 2† | |||
| IRF8+/+ HSCs | 21.3 | 10.3 | 26.0 |
| IRF8−/− HSCs | 35.2 | 15.8 | 35.2 |
HSCs were sorted and transfected with vectors expressing eGFP-IRF8, eGFP-K79E, or eGFP alone and were resorted for GFP+ cells. Cells (1 × 104) were cultured in 24-well plate containing OP9 cells as well as B-cell permissive cytokines SCF, IL-7, and Flt3L for 5 days.
GFP+ cells (3-6 × 104) were cultured at the same conditions as in Expt 1 for 4 days.