Phenotypical analysis of SAC-activated B cells after 3-day culture
% . | CD25 . | CD69 . | CD80 . | CD86 . | CD54 . | B7-H1 . | CD95 . | CD38 . | CD27 . |
---|---|---|---|---|---|---|---|---|---|
B large | 91 ± 6 | 33 ± 9 | 82 ± 7 | 85 ± 5 | 71 ± 8 | 45 ± 11 | 61 ± 15 | 74 ± 7 | 29 ± 6 |
B small | 15 ± 4 | 3 ± 2 | 22 ± 6 | 22 ± 9 | 17 ± 5 | 23 ± 6 | 12 ± 4 | 13 ± 4 | 18 ± 6 |
% . | CD25 . | CD69 . | CD80 . | CD86 . | CD54 . | B7-H1 . | CD95 . | CD38 . | CD27 . |
---|---|---|---|---|---|---|---|---|---|
B large | 91 ± 6 | 33 ± 9 | 82 ± 7 | 85 ± 5 | 71 ± 8 | 45 ± 11 | 61 ± 15 | 74 ± 7 | 29 ± 6 |
B small | 15 ± 4 | 3 ± 2 | 22 ± 6 | 22 ± 9 | 17 ± 5 | 23 ± 6 | 12 ± 4 | 13 ± 4 | 18 ± 6 |
Data represent mean plus or minus SD from purified B cells of 3 donors after 3-day culture with SAC and IL-2. PI+ cells were excluded and for analysis 2 gates were set according to cell size.