Table 1 The cell subsets comprising... | American Society of Hematology

Table 1

The cell subsets comprising GFP-tg APCs

Cell subset	Surface markers	% in APCs, plus and minus 1 SD*	% GFP⁺	No. of injected cells × 10³†
B cells	B220⁺ CD19⁺	84.4 ± 2.7	81.4	12659
Lymphoid DCs	CD11c⁺ CD11b⁻ CD8α⁺	1.5 ± 0.3	97.7	229
CD45RB^hi DCs	CD11c^low CD45RB^hi	0.8 ± 0.1	85.2	121
Myeloid DCs	CD11c⁺ CD11b⁺ CD8α⁻	0.5 ± 0.01	91.7	73
Plasmacytoid DCs	CD11c⁺ B220⁺ GR-1⁺	0.3 ± 0.02	92.8	48
NK cells	NK1.1⁺	2.3 ± 0.4	90.9	352
Monocytes/MΦ	CD14⁺	2.0 ± 0.2	81.4	303
CD4⁺ T cells	CD3⁺ CD4⁺	1.5 ± 0.4	83.6	228
Granulocytes	B220⁻ GR1⁺	1.4 ± 0.7	88.4	211
CD8⁺ T cells	CD3⁺ CD8⁺	0.3 ± 0.003	80.4	51
NK T cells	NK1.1⁺ TCRβ⁺	0.1 ± 0.002	81.6	20

Cell subset	Surface markers	% in APCs, plus and minus 1 SD*	% GFP⁺	No. of injected cells × 10³†
B cells	B220⁺ CD19⁺	84.4 ± 2.7	81.4	12659
Lymphoid DCs	CD11c⁺ CD11b⁻ CD8α⁺	1.5 ± 0.3	97.7	229
CD45RB^hi DCs	CD11c^low CD45RB^hi	0.8 ± 0.1	85.2	121
Myeloid DCs	CD11c⁺ CD11b⁺ CD8α⁻	0.5 ± 0.01	91.7	73
Plasmacytoid DCs	CD11c⁺ B220⁺ GR-1⁺	0.3 ± 0.02	92.8	48
NK cells	NK1.1⁺	2.3 ± 0.4	90.9	352
Monocytes/MΦ	CD14⁺	2.0 ± 0.2	81.4	303
CD4⁺ T cells	CD3⁺ CD4⁺	1.5 ± 0.4	83.6	228
Granulocytes	B220⁻ GR1⁺	1.4 ± 0.7	88.4	211
CD8⁺ T cells	CD3⁺ CD8⁺	0.3 ± 0.003	80.4	51
NK T cells	NK1.1⁺ TCRβ⁺	0.1 ± 0.002	81.6	20

Averages of 3 animals are shown in columns 3, 4, and 5. Plus and minus 1 SD is included in column 3.

Calculated on R1 gate that excludes red blood cells (R₁ = 97.75% of the total events acquired).

Calculated on a total of 15 × 10⁶ APCs injected per mouse.