Generation of complex repertoires in the peripheral blood early after transplantation
| Input (mouse) . | A . | B . | C . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BV families skewed* | 15 | 17 | 16 | |||||||||
| Day after BMT | 38 | 66 | 38 | 66 | 38 | 66 | ||||||
| Recipient mouse | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 |
| Degree of reconstitution† | ||||||||||||
| None | 1 | 1 | 0 | 0 | 4 | 4 | 0 | 0 | 3 | 3 | 0 | 0 |
| Partial | 10 | 5 | 2 | 2 | 6 | 6 | 6 | 7 | 3 | 4 | 3 | 2 |
| Complete | 7 | 12 | 10 | 13 | 9 | 8 | 12 | 11 | 3 | 5 | 13 | 13 |
| Loss | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 |
| New skew | 0 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 8 | 5 | 2 | 1 |
| Input (mouse) . | A . | B . | C . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BV families skewed* | 15 | 17 | 16 | |||||||||
| Day after BMT | 38 | 66 | 38 | 66 | 38 | 66 | ||||||
| Recipient mouse | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 |
| Degree of reconstitution† | ||||||||||||
| None | 1 | 1 | 0 | 0 | 4 | 4 | 0 | 0 | 3 | 3 | 0 | 0 |
| Partial | 10 | 5 | 2 | 2 | 6 | 6 | 6 | 7 | 3 | 4 | 3 | 2 |
| Complete | 7 | 12 | 10 | 13 | 9 | 8 | 12 | 11 | 3 | 5 | 13 | 13 |
| Loss | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 |
| New skew | 0 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 8 | 5 | 2 | 1 |
Lethally irradiated (1100 cGy) AKR mice were transplanted with Rag-1 BM (5 × 106) and a spleen cell-adjusted dose of 5 × 105 T cells obtained 19 to 29 days after BMT from B6→AKR chimeras. Spleen cells from individual chimeras were each then transplanted into 2 to 3 secondary AKR hosts. AKR animals that received a secondary transplant were bled 38 and 66 days after BMT, and peripheral blood cells were analyzed by CDR3 spectratyping.
The number of BV families that were skewed from input splenocytes. A total of 19 families were examined in all animals.
Reconstitution of BV families was graded as none, partial, or complete, using the same criteria as noted in the legend to Table 1 Loss refers to absence of a spectratype that was attributed to technical failure. Consequently, the total number of BV families at each time point for each animal does not total 19 families in all cases. New skew refers to the emergence of skewed bands that were not present in the input spleen cell populations from primary GVHD animals.