Disease characteristics of mice that received a transplant of young or old EGFP or BCR-ABLP210–transduced BM cells in one representative experiment
| Donor . | BM . | Spleen . | Disease type . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Total cells, × 106* . | GFP+, % . | GFP+ Gr-1+ CD11b+, % . | GFP+ CD45R+ CD19+, % . | Total cells, × 106 . | GFP+, % . | GFP+ Gr-1+ CD11b+, % . | GFP+ CD45R+ CD19+, % . | ||
| Young | |||||||||
| EGFP1 | 9.4 | 1.9 | 1.1 | 0.6 | 15.8 | 5.9 | 2.8 | 0.9 | None |
| EGFP2 | 26.0 | 1.5 | 0.8 | 0.2 | 52.8 | 1.8 | 0.5 | 1.3 | None |
| EGFP3 | 13.2 | 2.1 | 0.9 | 0.3 | 48.0 | 2.5 | 0.4 | 1.7 | None |
| EGFP4 | 18.8 | 1.9 | 0.9 | 0.3 | 61.6 | 5.3 | 0.3 | 1.5 | None |
| BCR1 | 9.2 | 62.0 | 39.1 | 0.2 | 52.8 | 75.3 | 55.1 | 1.0 | M |
| BCR2 | N/A | 64.1 | 12.7 | 2.5 | NA | 84.8 | 16.5 | 1.3 | M |
| BCR3 | 11.0 | 76.3 | 52.3 | 0.3 | 90.2 | 86.7 | 42.8 | 0.9 | M |
| BCR4 | 10.2 | 65.4 | 25.7 | 0.1 | 92.2 | 76.6 | 50.0 | 0.7 | M |
| BCR5 | 12.0 | 55.7 | 44.7 | 5.2 | 108.5 | 66.0 | 38.9 | 6.4 | M + B |
| BCR6 | 7.2 | 32.0 | 10.9 | 19.5 | 130.4 | 74.5 | 9.9 | 15.1 | M + B |
| BCR7 | 11.6 | 60.7 | 1.3 | 58.8 | 56.8 | 58.6 | 5.2 | 48.4 | B |
| BCR8 | 10.2 | 82.0 | 56.9 | 0.5 | 199.2 | 56.0 | 37.6 | 2.9 | M |
| Old | |||||||||
| EGFP1 | 17.8 | 2.4 | 1.7 | 0.1 | 72.0 | 1.2 | 0.7 | 0.4 | None |
| EGFP2 | 23.4 | 1.4 | 1.0 | 0.2 | 82.6 | 2.2 | 1.4 | 0.3 | None |
| BCR1 | 8.8 | 68.9 | 27.5 | 0.2 | 148.8 | 65.6 | 18.2 | 2.6 | M |
| BCR2 | 20.0 | 17.8 | 12.9 | 0.1 | 136.3 | 43.7 | 12.8 | 1.2 | M |
| BCR3 | 15.6 | 34.4 | 26.6 | 0.2 | 144.0 | 58.4 | 32.2 | 1.7 | M |
| BCR4 | 9.4 | 56.0 | 23.1 | 0.1 | 168.0 | 53.8 | 21.0 | 2.2 | M |
| BCR5 | 15.2 | 45.4 | 24.0 | 0.1 | 178.6 | 62.4 | 19.5 | 1.2 | M |
| BCR6 | 14.8 | 30.7 | 25.2 | 0.1 | 121.0 | 50.7 | 26.5 | 1.8 | M |
| BCR7 | 20.6 | 22.7 | 18.4 | 0.1 | 136.3 | 52.6 | 20.3 | 1.3 | M |
| BCR8 | 20.8 | 2.2 | 1.6 | 0.1 | 67.2 | 21.7 | 9.0 | 0.7 | M |
| Donor . | BM . | Spleen . | Disease type . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Total cells, × 106* . | GFP+, % . | GFP+ Gr-1+ CD11b+, % . | GFP+ CD45R+ CD19+, % . | Total cells, × 106 . | GFP+, % . | GFP+ Gr-1+ CD11b+, % . | GFP+ CD45R+ CD19+, % . | ||
| Young | |||||||||
| EGFP1 | 9.4 | 1.9 | 1.1 | 0.6 | 15.8 | 5.9 | 2.8 | 0.9 | None |
| EGFP2 | 26.0 | 1.5 | 0.8 | 0.2 | 52.8 | 1.8 | 0.5 | 1.3 | None |
| EGFP3 | 13.2 | 2.1 | 0.9 | 0.3 | 48.0 | 2.5 | 0.4 | 1.7 | None |
| EGFP4 | 18.8 | 1.9 | 0.9 | 0.3 | 61.6 | 5.3 | 0.3 | 1.5 | None |
| BCR1 | 9.2 | 62.0 | 39.1 | 0.2 | 52.8 | 75.3 | 55.1 | 1.0 | M |
| BCR2 | N/A | 64.1 | 12.7 | 2.5 | NA | 84.8 | 16.5 | 1.3 | M |
| BCR3 | 11.0 | 76.3 | 52.3 | 0.3 | 90.2 | 86.7 | 42.8 | 0.9 | M |
| BCR4 | 10.2 | 65.4 | 25.7 | 0.1 | 92.2 | 76.6 | 50.0 | 0.7 | M |
| BCR5 | 12.0 | 55.7 | 44.7 | 5.2 | 108.5 | 66.0 | 38.9 | 6.4 | M + B |
| BCR6 | 7.2 | 32.0 | 10.9 | 19.5 | 130.4 | 74.5 | 9.9 | 15.1 | M + B |
| BCR7 | 11.6 | 60.7 | 1.3 | 58.8 | 56.8 | 58.6 | 5.2 | 48.4 | B |
| BCR8 | 10.2 | 82.0 | 56.9 | 0.5 | 199.2 | 56.0 | 37.6 | 2.9 | M |
| Old | |||||||||
| EGFP1 | 17.8 | 2.4 | 1.7 | 0.1 | 72.0 | 1.2 | 0.7 | 0.4 | None |
| EGFP2 | 23.4 | 1.4 | 1.0 | 0.2 | 82.6 | 2.2 | 1.4 | 0.3 | None |
| BCR1 | 8.8 | 68.9 | 27.5 | 0.2 | 148.8 | 65.6 | 18.2 | 2.6 | M |
| BCR2 | 20.0 | 17.8 | 12.9 | 0.1 | 136.3 | 43.7 | 12.8 | 1.2 | M |
| BCR3 | 15.6 | 34.4 | 26.6 | 0.2 | 144.0 | 58.4 | 32.2 | 1.7 | M |
| BCR4 | 9.4 | 56.0 | 23.1 | 0.1 | 168.0 | 53.8 | 21.0 | 2.2 | M |
| BCR5 | 15.2 | 45.4 | 24.0 | 0.1 | 178.6 | 62.4 | 19.5 | 1.2 | M |
| BCR6 | 14.8 | 30.7 | 25.2 | 0.1 | 121.0 | 50.7 | 26.5 | 1.8 | M |
| BCR7 | 20.6 | 22.7 | 18.4 | 0.1 | 136.3 | 52.6 | 20.3 | 1.3 | M |
| BCR8 | 20.8 | 2.2 | 1.6 | 0.1 | 67.2 | 21.7 | 9.0 | 0.7 | M |
NA indicates not applicable; M, myeloid; B, lymphoid.
Numbers of BM cells are from 2 femurs and 2 tibias.