Identification of the ED2 antigen as rat CD163
| Peptide . | ED2 antigen . | Rat CD163 . | Position . | Identity, % . |
|---|---|---|---|---|
| 1* | VTQAPEGRKKELLLAG | VTQAPEGRKKELRLAG | 30-45 | 94 |
| 2 | WGTVCDDNFSK | WGTVCDDNFSK | 170-180 | 100 |
| 3 | QLGCGSALSFSGSAK | QLGCGSALSFSGSAK | 189-203 | 100 |
| 4 | QLGCPTAITAIGRVNASK | QLGCPTAITAIGRVNASE | 300-318 | 94 |
| 5 | EDAGVTCSDGADLELR | EDAGVTCSDGADLELR | 349-364 | 100 |
| 6 | LVGGEIPCSGR | LVGGEIPCSGR | 475-485 | 100 |
| 7 | GAGQVWRHK | GAGQVWRHK | 633-641 | 100 |
| 8 | VDTLWQCPSSPWK | VDTLWQCPSSPWK | 894-906 | 100 |
| 9 | EAAFGPGTGPIWLNEMK | EAAFGPGTGPIWLNEMK | 976-992 | 100 |
| Peptide . | ED2 antigen . | Rat CD163 . | Position . | Identity, % . |
|---|---|---|---|---|
| 1* | VTQAPEGRKKELLLAG | VTQAPEGRKKELRLAG | 30-45 | 94 |
| 2 | WGTVCDDNFSK | WGTVCDDNFSK | 170-180 | 100 |
| 3 | QLGCGSALSFSGSAK | QLGCGSALSFSGSAK | 189-203 | 100 |
| 4 | QLGCPTAITAIGRVNASK | QLGCPTAITAIGRVNASE | 300-318 | 94 |
| 5 | EDAGVTCSDGADLELR | EDAGVTCSDGADLELR | 349-364 | 100 |
| 6 | LVGGEIPCSGR | LVGGEIPCSGR | 475-485 | 100 |
| 7 | GAGQVWRHK | GAGQVWRHK | 633-641 | 100 |
| 8 | VDTLWQCPSSPWK | VDTLWQCPSSPWK | 894-906 | 100 |
| 9 | EAAFGPGTGPIWLNEMK | EAAFGPGTGPIWLNEMK | 976-992 | 100 |
Peptide sequences of the rat ED2 antigen and comparison (ie, position, percent amino acid identity) to corresponding sequence from the predicted rat CD163 (National Center for Biotechnology Information Sequence Viewer; http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&=62648134). Differences between the determined and predicted sequence are underlined. (An alignment of rat, mouse, and human CD163 is provided in Figure S1.)
N = terminal.