Table 1

Kinetic determination of VWF binding to ADAMTS13 (or variants) by SPR

VWF; KD, ×10−9 M (n)VWF;*KD ×10−9 M (n)
FL-A13  
    50±9 (6) 83 ± 17 (6) 
    77±26 (4) — 
delCUB  
    274±92 (6) 242 ± 73 (6) 
    468±131 (4) — 
MDTC  
    No binding 337 ± 186 (6) 
    No binding — 
VWF; KD, ×10−9 M (n)VWF;*KD ×10−9 M (n)
FL-A13  
    50±9 (6) 83 ± 17 (6) 
    77±26 (4) — 
delCUB  
    274±92 (6) 242 ± 73 (6) 
    468±131 (4) — 
MDTC  
    No binding 337 ± 186 (6) 
    No binding — 

The entries are means plus and minus a standard error.

FL-A13 is the full-length ADAMTS13; delCUB, the ADAMTS13 variant truncated after the eighth TSP1 repeat; MDTCS, the variant truncated after the spacer domain; n, the number of repeats performed; —, not applicable.

*

The VWF substrate was denatured at 37°C for 2 hours with 1.5 M quanidine HCl prior to binding experiments.

Data obtained from experiments performed in presence of 10 mM EDTA.

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