Kinetic determination of VWF binding to ADAMTS13 (or variants) by SPR
| VWF; KD, ×10−9 M (n) . | VWF;*KD ×10−9 M (n) . |
|---|---|
| FL-A13 | |
| 50±9 (6) | 83 ± 17 (6) |
| 77±26 (4)† | — |
| delCUB | |
| 274±92 (6) | 242 ± 73 (6) |
| 468±131 (4)† | — |
| MDTC | |
| No binding | 337 ± 186 (6) |
| No binding† | — |
| VWF; KD, ×10−9 M (n) . | VWF;*KD ×10−9 M (n) . |
|---|---|
| FL-A13 | |
| 50±9 (6) | 83 ± 17 (6) |
| 77±26 (4)† | — |
| delCUB | |
| 274±92 (6) | 242 ± 73 (6) |
| 468±131 (4)† | — |
| MDTC | |
| No binding | 337 ± 186 (6) |
| No binding† | — |
The entries are means plus and minus a standard error.
FL-A13 is the full-length ADAMTS13; delCUB, the ADAMTS13 variant truncated after the eighth TSP1 repeat; MDTCS, the variant truncated after the spacer domain; n, the number of repeats performed; —, not applicable.
The VWF substrate was denatured at 37°C for 2 hours with 1.5 M quanidine HCl prior to binding experiments.
Data obtained from experiments performed in presence of 10 mM EDTA.