RV integration site distribution in human CD34+ HSCs
| . | Intergenic, % . | Intragenic, % . | Perigenic, % . | Total hits . | Within 10 kb from TSS, % . | Total vector/gene interactions* . |
|---|---|---|---|---|---|---|
| CD34+ cells | ||||||
| RV vectors | 16.7 | 55.0 | 28.3 | 1030 | 29.3 | 1517 |
| LV vectors | 17.4 | 71.7 | 10.9 | 849 | 16.1 | 1241 |
| Control sequences | 46.2 | 38.6 | 15.2 | 798 | 9.1 | 902 |
| RV hot spots | 16.0 | 56.6 | 27.4 | 219 | 22.2 | 302 |
| LV hot spots | 8.6 | 81.4 | 10.0 | 70 | 13.2 | 2114 |
| Control hot spots | 36.4 | 59.1 | 4.5 | 22 | 13.0 | 23 |
| HeLa cells | ||||||
| RV vectors | 26.4 | 48.1 | 25.5 | 869 | 26.1 | 1219 |
| RV hot spots | 16.5 | 53.2 | 30.3 | 109 | 27.3 | 165 |
| . | Intergenic, % . | Intragenic, % . | Perigenic, % . | Total hits . | Within 10 kb from TSS, % . | Total vector/gene interactions* . |
|---|---|---|---|---|---|---|
| CD34+ cells | ||||||
| RV vectors | 16.7 | 55.0 | 28.3 | 1030 | 29.3 | 1517 |
| LV vectors | 17.4 | 71.7 | 10.9 | 849 | 16.1 | 1241 |
| Control sequences | 46.2 | 38.6 | 15.2 | 798 | 9.1 | 902 |
| RV hot spots | 16.0 | 56.6 | 27.4 | 219 | 22.2 | 302 |
| LV hot spots | 8.6 | 81.4 | 10.0 | 70 | 13.2 | 2114 |
| Control hot spots | 36.4 | 59.1 | 4.5 | 22 | 13.0 | 23 |
| HeLa cells | ||||||
| RV vectors | 26.4 | 48.1 | 25.5 | 869 | 26.1 | 1219 |
| RV hot spots | 16.5 | 53.2 | 30.3 | 109 | 27.3 | 165 |
Distribution of RV and LV integration sites unambiguously mapped in unselected CB- and BM-derived CD34+ HSCs, and RV integrations in HeLa cells from a previously published collection.27 . Integrations (total hits) were distributed as inside (intragenic), outside (intergenic), or at a distance of less than 30 kb upstream or dowstream (perigenic) from known genes (UCSC annotation). Insertions at a distance of ±10 kb from transcription start sites (TSSs) are indicated as percentage of the total vector/gene interactions. Control sequences were obtained from a randomly cloned library of Pst1/MseI-restricted, LM-PCR–amplified human CD34+ cell DNA.
Total number of genes within 30 kb from individual hits + intergenic hits.