Table 2 Functional analyses of sorted... | American Society of Hematology

Table 2

Functional analyses of sorted CD34⁺ sub-fractions in LTC-IC assays

LTC-IC frequency	n	CD34′, %	CD133⁺, %	CD133^low/−, %	P CD133⁺ to CD133^low/−	P CD133⁺ to CD34⁺
CD38	3	4.2 ± 3.5	CD38^low, 13.4 ± 1.8	CD38⁺, 0.3 ± 0.4	< .01	< .02
CD53	5	3.4 ± 2.7	CD53⁺, 7.9 ± 4.2	CD53^low/−, 1.1 ± 1.0	< .03	< .03
CD62L	5	3.4 ± 2.7	CD62L⁺, 8.9 ± 3.9	CD62L^low/−, 0.3 ± 0.1	< .01	< .01
CD63	5	5.3 ± 2.8	CD63^low/−, 11.4 ± 3.0	CD63⁺, 0.3 ± 0.2	< .01	< .01
CD71	5	3.4 ± 2.7	CD71^low, 12.7 ± 10.0	CD71⁺, 1.0 ± 0.8	< .06	< .05

LTC-IC frequency	n	CD34′, %	CD133⁺, %	CD133^low/−, %	P CD133⁺ to CD133^low/−	P CD133⁺ to CD34⁺
CD38	3	4.2 ± 3.5	CD38^low, 13.4 ± 1.8	CD38⁺, 0.3 ± 0.4	< .01	< .02
CD53	5	3.4 ± 2.7	CD53⁺, 7.9 ± 4.2	CD53^low/−, 1.1 ± 1.0	< .03	< .03
CD62L	5	3.4 ± 2.7	CD62L⁺, 8.9 ± 3.9	CD62L^low/−, 0.3 ± 0.1	< .01	< .01
CD63	5	5.3 ± 2.8	CD63^low/−, 11.4 ± 3.0	CD63⁺, 0.3 ± 0.2	< .01	< .01
CD71	5	3.4 ± 2.7	CD71^low, 12.7 ± 10.0	CD71⁺, 1.0 ± 0.8	< .06	< .05

n indicates number of experiments; CD34⁺, LTC-IC frequency of CD34⁺ cells purified at culture day 3 (note: identical CB- samples for CD53, CD62L, and CD71 analyses were used); CD133⁺, LTC-IC frequency of purified, cultured CD34⁺ CD133⁺ cell fractions which are CD38^low, CD53⁺, CD62L⁺, CD63^low/−, or CD71^low, respectively; CD133^low, LTC-IC frequency of purified, cultured CD34⁺ CD133^low/− cell fractions which are CD38⁺, CD53^low/−, CD62L^low/−, CD63⁺, or CD71⁺, respectively; P CD133⁺ to CD133^low/−, P values of significance analyses of the LTC-IC frequency of CD34⁺CD133⁺ cell fractions compared with that of corresponding CD34⁺CD133^low/− cell fractions; P CD133⁺ to CD34⁺, P values of significance analyses of the LTC-IC frequency of CD34⁺CD133⁺ cell fractions compared with that of corresponding CD34⁺ cell fractions.

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