The effect of inhibitors on platelet adhesion to fibrinogen
| Inhibitor . | Dose . | Target . | Platelet adhesion, % of control . | |
|---|---|---|---|---|
| Low-density fibrinogen . | High-density fibrinogen . | |||
| Bisindolylmaleimide | 10 μM | PKC | 49 ± 15* | 85 ± 2* |
| Wortmannin | 20 nM | PI3K | 51 ± 15* | 84 ± 14 |
| PP2 | 20 μM | Src kinase | 43 ± 9* | 99 ± 10 |
| Cytochalasin D | 10 μM | Actin polymerization | 20 ± 14* | 104 ± 5 |
| Apyrase | 2 U/mL | ADP | 57 ± 12* | 86 ± 7* |
| H-1152 | 5 μM | Rho kinase | 103 ± 19 | 100 ± 9 |
| NSC23766 | 100 μM | Rac1 | 82 ± 14 | 103 ± 4 |
| Inhibitor . | Dose . | Target . | Platelet adhesion, % of control . | |
|---|---|---|---|---|
| Low-density fibrinogen . | High-density fibrinogen . | |||
| Bisindolylmaleimide | 10 μM | PKC | 49 ± 15* | 85 ± 2* |
| Wortmannin | 20 nM | PI3K | 51 ± 15* | 84 ± 14 |
| PP2 | 20 μM | Src kinase | 43 ± 9* | 99 ± 10 |
| Cytochalasin D | 10 μM | Actin polymerization | 20 ± 14* | 104 ± 5 |
| Apyrase | 2 U/mL | ADP | 57 ± 12* | 86 ± 7* |
| H-1152 | 5 μM | Rho kinase | 103 ± 19 | 100 ± 9 |
| NSC23766 | 100 μM | Rac1 | 82 ± 14 | 103 ± 4 |
Platelets were allowed to adhere to fibrinogen coated microtiter wells for 1 hour. After washing, platelet adhesion was assessed by endogenous acid phosphatase activity. Adhesion is expressed as a percentage of control adhesion and results are presented as mean ± SD (n = 3).
P < .05 compared to control.