Reversibility of platelet adhesion by αIIbβ3 antagonists or EDTA
| Inhibitor . | Concentration . | Residual platelet adhesion, % of control . | n . | |
|---|---|---|---|---|
| Low-density fibrinogen . | High-density fibrinogen . | |||
| c7E3 Fab | 10 μg/mL | 75 ± 3 | 34 ± 13* | 3 |
| EDTA | 10 mM | 66 ± 14 | 43 ± 21* | 7 |
| Tirofiban | 100 μM | 69 ± 21 | 37 ± 25* | 4 |
| Inhibitor . | Concentration . | Residual platelet adhesion, % of control . | n . | |
|---|---|---|---|---|
| Low-density fibrinogen . | High-density fibrinogen . | |||
| c7E3 Fab | 10 μg/mL | 75 ± 3 | 34 ± 13* | 3 |
| EDTA | 10 mM | 66 ± 14 | 43 ± 21* | 7 |
| Tirofiban | 100 μM | 69 ± 21 | 37 ± 25* | 4 |
Platelets were allowed to adhere to fibrinogen-coated microtiter wells for 1 hour, unbound platelets were removed by washing, and wells were further filled with buffer (control) or one of the inhibitors for 1 hour. After washing, the residual platelet adhesion was assessed by measuring endogenous acid phosphatase activity and expressed as a percentage of platelets adherent in the control wells.
Results are expressed as mean ± SD.
P ≤ .01 compared to low-density fibrinogen.