Oligo sequences
| Gene product . | Gene symbol . | Forward primer . | Reverse primer . | Accession no. . |
|---|---|---|---|---|
| CCAAT/enhancer binding protein-α | Cebpa | 5′-agagccgagataagccaaa-3′ | 5′-ggtcaactccagcaccttct-3′ | NM_007678 |
| Ferritin heavy chain 1 | Fth1 | 5′-aagaaaccagaccgtgatga-3′ | 5′-tagccagtttgtgcagttcc-3′ | NM_010239 |
| Ferritin light chain 1 | Ftl1 | 5′-ctcatcaagaagatgggcaa-3′ | 5′-gagatactcgcccagagacc-3′ | NM_010240 |
| Hemochromatosis | Hfe | 5′-gcaatctcaggccatgatta-3′ | 5′-attccaaccaagaagatggc-3′ | NM_010424 |
| Hemochromatosis type 2 (juvenile) | Hjv, Hfe2 | 5′-gctcgagtttgtccattcaa-3′ | 5′-tgttccaatgtaggcagctc-3′ | NM_027126 |
| Hepcidin antimicrobial peptide 1 | Hamp, Hamp1 | 5′-tgagcagcaccacctatctc-3′ | 5′-acagcagaagatgcagatgg-3′ | NM_032541 |
| Solute carrier family 11 | Dmt1, Slc11a2 | 5′-tgtttgattgcattgggtctg-3′ | 5′-cgctcagcaggactttcgag-3′ | BC019137 |
| Solute carrier family 40 | Fpn1, Slc40a1 | 5′-caagaatgagctcctgacca-3′ | 5′-gccacaacaacaatccagtc-3′ | NM_016917 |
| Transferrin | Tf, Trf | 5′-cttctcatgctgttgtggct-3′ | 5′-gttcctgtgccactttgaga-3′ | NM_133977 |
| Transferrin receptor | Tfr1, Tfrc | 5′-cagaccttgcactctttgga-3′ | 5′-gaaagaaggaaagccaggtg-3′ | NM_011638 |
| Transferrin receptor 2 | Tfr2, Trfr2 | 5′-gggttcagacctcgaagaag-3′ | 5′-aggctcacgtacacaacagc-3′ | NM_015799 |
| β-actin | Actb | 5′-ccttccttcttgggtatgga-3′ | 5′-acggatgtcaacgtcacact-3′ | NM_007393 |
| Gene product . | Gene symbol . | Forward primer . | Reverse primer . | Accession no. . |
|---|---|---|---|---|
| CCAAT/enhancer binding protein-α | Cebpa | 5′-agagccgagataagccaaa-3′ | 5′-ggtcaactccagcaccttct-3′ | NM_007678 |
| Ferritin heavy chain 1 | Fth1 | 5′-aagaaaccagaccgtgatga-3′ | 5′-tagccagtttgtgcagttcc-3′ | NM_010239 |
| Ferritin light chain 1 | Ftl1 | 5′-ctcatcaagaagatgggcaa-3′ | 5′-gagatactcgcccagagacc-3′ | NM_010240 |
| Hemochromatosis | Hfe | 5′-gcaatctcaggccatgatta-3′ | 5′-attccaaccaagaagatggc-3′ | NM_010424 |
| Hemochromatosis type 2 (juvenile) | Hjv, Hfe2 | 5′-gctcgagtttgtccattcaa-3′ | 5′-tgttccaatgtaggcagctc-3′ | NM_027126 |
| Hepcidin antimicrobial peptide 1 | Hamp, Hamp1 | 5′-tgagcagcaccacctatctc-3′ | 5′-acagcagaagatgcagatgg-3′ | NM_032541 |
| Solute carrier family 11 | Dmt1, Slc11a2 | 5′-tgtttgattgcattgggtctg-3′ | 5′-cgctcagcaggactttcgag-3′ | BC019137 |
| Solute carrier family 40 | Fpn1, Slc40a1 | 5′-caagaatgagctcctgacca-3′ | 5′-gccacaacaacaatccagtc-3′ | NM_016917 |
| Transferrin | Tf, Trf | 5′-cttctcatgctgttgtggct-3′ | 5′-gttcctgtgccactttgaga-3′ | NM_133977 |
| Transferrin receptor | Tfr1, Tfrc | 5′-cagaccttgcactctttgga-3′ | 5′-gaaagaaggaaagccaggtg-3′ | NM_011638 |
| Transferrin receptor 2 | Tfr2, Trfr2 | 5′-gggttcagacctcgaagaag-3′ | 5′-aggctcacgtacacaacagc-3′ | NM_015799 |
| β-actin | Actb | 5′-ccttccttcttgggtatgga-3′ | 5′-acggatgtcaacgtcacact-3′ | NM_007393 |
Primer design was performed using Primer Express Software version 2.0 (Applied Biosystems), based on the corresponding mouse gene sequences obtained from PubMed. As endogenous controls to normalize gene expression levels, a mouse Gapdh VIC/MGB probe (Applied Biosystems) and mouse β-actin were used. Two endogenous control genes were used to determine if any difference in amplification existed due to the effect of iron overload.