Real-time PCR analysis and VEGF secretion on 12 primary AML samples and AML cell lines
| Case . | FAB . | hERG1 . | VEGF (pg/ml) . | |
|---|---|---|---|---|
| 1A . | 1B . | |||
| PBMNC | / | 1.00 | 1.00 | n.d. |
| A17 | M1 | 18.00 | 68.10 | 175 ± 4 |
| A21 | M1 | 10.40 | 7.94 | 85 ± 7 |
| A50 | M1 | 190.00 | 70.60 | 148 ± 14 |
| A16 | M2 | 0.43 | 0.70 | 66 ± 13 |
| A25 | M2 | 3.28 | 0.55 | 71 ± 13 |
| A60 | M2 | 0.05 | 0.35 | 59 ± 2 |
| A57 | M2 | 9.40 | 238.00 | 124 ± 11 |
| A29 | M3 | 4.22 | 562000.00 | 144 ± 12 |
| A4 | M4 | 107.00 | 20.10 | 183 ± 6 |
| A48 | M4 | 12.20 | 0.92 | 125 ± 6 |
| A64 | M6 | 6.98 | 30.20 | 85 ± 4 |
| A1 | M4 | 0.85 | 0.44 | 77 ± 2 |
| FLG | M5 | 280.00 | 6840.00 | 358 ± 24 |
| FLG+Way | M5 | n.d. | n.d. | 237 ± 16 |
| K562 | Acute phase of CML | 10.10 | 480.00 | 99 ± 8 |
| NB4 | M3 | 27.80 | 660.00 | 253 ± 14 |
| HL60 | M3 | 13.70 | 94.70 | 132 ± 8 |
| hERG1-HL60 | M3 | 224.00 | 98.10 | 292 ± 21 |
| Case . | FAB . | hERG1 . | VEGF (pg/ml) . | |
|---|---|---|---|---|
| 1A . | 1B . | |||
| PBMNC | / | 1.00 | 1.00 | n.d. |
| A17 | M1 | 18.00 | 68.10 | 175 ± 4 |
| A21 | M1 | 10.40 | 7.94 | 85 ± 7 |
| A50 | M1 | 190.00 | 70.60 | 148 ± 14 |
| A16 | M2 | 0.43 | 0.70 | 66 ± 13 |
| A25 | M2 | 3.28 | 0.55 | 71 ± 13 |
| A60 | M2 | 0.05 | 0.35 | 59 ± 2 |
| A57 | M2 | 9.40 | 238.00 | 124 ± 11 |
| A29 | M3 | 4.22 | 562000.00 | 144 ± 12 |
| A4 | M4 | 107.00 | 20.10 | 183 ± 6 |
| A48 | M4 | 12.20 | 0.92 | 125 ± 6 |
| A64 | M6 | 6.98 | 30.20 | 85 ± 4 |
| A1 | M4 | 0.85 | 0.44 | 77 ± 2 |
| FLG | M5 | 280.00 | 6840.00 | 358 ± 24 |
| FLG+Way | M5 | n.d. | n.d. | 237 ± 16 |
| K562 | Acute phase of CML | 10.10 | 480.00 | 99 ± 8 |
| NB4 | M3 | 27.80 | 660.00 | 253 ± 14 |
| HL60 | M3 | 13.70 | 94.70 | 132 ± 8 |
| hERG1-HL60 | M3 | 224.00 | 98.10 | 292 ± 21 |
The numbers represent the transcript levels of herg1a and herg1b in primary leukemia samples and AML cell lines, standardized to the levels in normal PB mononuclear cells from 6 donors. VEGF was measured by enzyme-linked immunosorbent assay (ELISA) test on the supernatants of cells cultured in serum free medium for 48 hours (“Patients, materials, and methods”). FLG 29.1 cells were treated or not with 40 μM Way throughout the entire time of the experiment as in Masi et al.28 hERG1-HL60 cells are HL60 cells stably transfected with the herg1 cDNA (“Patients, materials, and methods” and the legend for Figure 7). For case reference numbers in, see Table 1. A significant correlation emerged between herg1a expression and the amount of secreted VEGF in both primary AML blasts and AML cell lines (regression analysis, P = .002).