Cell counts of BM and of peripheral blood samples and clonogenic progenitor assay of Lin- BM cells from control and tPtch−/− mice
| . | Controls . | tPtch−/− (19 days) . | |||||
|---|---|---|---|---|---|---|---|
| Mean . | Deviation . | n . | Mean . | Deviation . | n . | P . | |
| CFU-GEMM | 0.6 | 0.8 | 5 | 0.5 | 0.5 | 4 | .811 |
| CFU-GM | 16.9 | 6.4 | 5 | 13.9 | 6.8 | 4 | .312 |
| CFU-G | 45.8 | 6.0 | 5 | 48.5 | 12.2 | 4 | .394 |
| CFU-M | 62.8 | 5.7 | 5 | 61.7 | 15.7 | 4 | .635 |
| BFU-E | 2.8 | 1.8 | 5 | 2.3 | 1.4 | 4 | .534 |
| . | Controls . | tPtch−/− (19 days) . | |||||
|---|---|---|---|---|---|---|---|
| Mean . | Deviation . | n . | Mean . | Deviation . | n . | P . | |
| CFU-GEMM | 0.6 | 0.8 | 5 | 0.5 | 0.5 | 4 | .811 |
| CFU-GM | 16.9 | 6.4 | 5 | 13.9 | 6.8 | 4 | .312 |
| CFU-G | 45.8 | 6.0 | 5 | 48.5 | 12.2 | 4 | .394 |
| CFU-M | 62.8 | 5.7 | 5 | 61.7 | 15.7 | 4 | .635 |
| BFU-E | 2.8 | 1.8 | 5 | 2.3 | 1.4 | 4 | .534 |
Progenitor activity of Lin- BM cells from tPtch−/−mice and from respective controls was examined 19 days after the onset of tamoxifen treatment using the colony-forming assay. Lin- BM cells (2 × 103) were cultured in methylcellulose-containing medium on a 35-mm dish. Lin- BM cells of each animal were tested in triplicates. Numbers of colonies/dish were counted after 8 days. Data for controls were pooled from both vehicle- and tamoxifen-treated animals.
n indicates the number of tested animals.