Primers for mRNA expression
| Primer . | Sequence . |
|---|---|
| VEGF164-S | GCC AGC ACA TAG AGA GAA TGA GC |
| VEGF164-AS | CAA GGC TCA CAG TGA TTT TCT GG |
| VEGF164-probe | TxRED-ACA GCA GAT GTG ATG GCA GAC CAA AGA AAG-BHQ2a |
| FLK-S | CGA CAT AGC CTC CAC TGT TTA TG |
| FLK-AS | TTT GTT CTT GTT CTC GGT GAT GT |
| FLK-probe | TET-TTC ATC GCC TCT GTC AGT GAC CAG CAT-BHQ1 |
| FLT-S | ACC TGT CCA ACT ACC TCA AGA GC |
| FLT-AS | CTG GTT CCA GGC TCT CTT TCT T |
| FLT-probe | TxRED-TTC TGT CTC AAC AAG GAC GCA GCC TT-BHQ2a |
| GAPDH-S | CCT GCA CCA CCA ACT GCT TA |
| GAPDH-AS | TCA TGA GCC CTT CCA CAA |
| GAPDH-probe | FAM-CCT GGC CAA GGT CAT CCA C-BHQ1 |
| Primer . | Sequence . |
|---|---|
| VEGF164-S | GCC AGC ACA TAG AGA GAA TGA GC |
| VEGF164-AS | CAA GGC TCA CAG TGA TTT TCT GG |
| VEGF164-probe | TxRED-ACA GCA GAT GTG ATG GCA GAC CAA AGA AAG-BHQ2a |
| FLK-S | CGA CAT AGC CTC CAC TGT TTA TG |
| FLK-AS | TTT GTT CTT GTT CTC GGT GAT GT |
| FLK-probe | TET-TTC ATC GCC TCT GTC AGT GAC CAG CAT-BHQ1 |
| FLT-S | ACC TGT CCA ACT ACC TCA AGA GC |
| FLT-AS | CTG GTT CCA GGC TCT CTT TCT T |
| FLT-probe | TxRED-TTC TGT CTC AAC AAG GAC GCA GCC TT-BHQ2a |
| GAPDH-S | CCT GCA CCA CCA ACT GCT TA |
| GAPDH-AS | TCA TGA GCC CTT CCA CAA |
| GAPDH-probe | FAM-CCT GGC CAA GGT CAT CCA C-BHQ1 |
S indicates sense; AS, antisense.