Effect of AZD1152 on clonogenic growth of freshly isolated leukemia cells
| Pt no. . | Age/sex . | FAB . | WBC count, ×109/L . | % blast . | Genetic abnormalities . | Source . | IC50 . | Previous treatment . |
|---|---|---|---|---|---|---|---|---|
| 1 | 62/F | L2 | 6.4 | 60 | Normal | PB | 2 | Yes |
| 2 | 47/F | M4 | 4.4 | 70 | Normal | PB | 3 | Yes |
| 3 | 64/M | M2 | 32.1 | 26 | Complex | BM | 3 | No |
| 4 | 32/M | L2 | 77.1 | 54 | t(9;22)(q34;q11) | PB | 1 | Yes |
| 5 | 68/F | CMLbc | 12 | 70 | t(9;22)(q34;q11) | BM | 2 | Yes |
| 6 | 71/M | M2 | 6.3 | 17 | Complex | PB | 2 | Yes |
| 7 | 51/F | M7 | 4.4 | 63 | t(12;15)(p13;q15) | PB | 3 | Yes |
| 8 | 59/M | M1 | 7.9 | 81 | der(1;7)(q10;q10) | PB | 3 | Yes |
| 9 | 64/F | M2 | 141 | 100 | Normal | PB | 3 | Yes |
| 10 | 59/M | M1 | 8.8 | 92 | Complex | PB | 2 | Yes |
| Pt no. . | Age/sex . | FAB . | WBC count, ×109/L . | % blast . | Genetic abnormalities . | Source . | IC50 . | Previous treatment . |
|---|---|---|---|---|---|---|---|---|
| 1 | 62/F | L2 | 6.4 | 60 | Normal | PB | 2 | Yes |
| 2 | 47/F | M4 | 4.4 | 70 | Normal | PB | 3 | Yes |
| 3 | 64/M | M2 | 32.1 | 26 | Complex | BM | 3 | No |
| 4 | 32/M | L2 | 77.1 | 54 | t(9;22)(q34;q11) | PB | 1 | Yes |
| 5 | 68/F | CMLbc | 12 | 70 | t(9;22)(q34;q11) | BM | 2 | Yes |
| 6 | 71/M | M2 | 6.3 | 17 | Complex | PB | 2 | Yes |
| 7 | 51/F | M7 | 4.4 | 63 | t(12;15)(p13;q15) | PB | 3 | Yes |
| 8 | 59/M | M1 | 7.9 | 81 | der(1;7)(q10;q10) | PB | 3 | Yes |
| 9 | 64/F | M2 | 141 | 100 | Normal | PB | 3 | Yes |
| 10 | 59/M | M1 | 8.8 | 92 | Complex | PB | 2 | Yes |
Leukemia cells were freshly isolated from peripheral blood or bone marrow of patients and were cultured in methylcellulose medium in the presence of cytokines with/without various concentrations of AZD1152 (0.3-10 nM). After 14 days, colonies were counted. Concentration of AZD1152 that induced 50% inhibition of colony formation (IC50) was calculated from the dose-response curves.
Pt indicates patient; FAB, French-American-British (leukemia classification); WBC, white blood cell; CMLbc, blast crisis of chronic myeloid leukemia; F, female; PB, peripheral blood; M, male; and BM, bone marrow.