Table 1 Oligonucleotide primers used for... | American Society of Hematology

Table 1

Oligonucleotide primers used for RT-PCR analysis

Genes	Primer sequences, 5′-3′		Annealing temp, °C	Cycle	Size, bp*
Genes	Forward	Reverse	Annealing temp, °C	Cycle	Size, bp*
Hprt	GCTGGTGAAAAGGACCTCT	CACAGGACTAGAACACCTGC	59	28 or QPCR	280
Alk1	TGCCTGTTCAGATGCCTTCAG	CTTGGGGAGCTTCAGAAGGGG	59	QPCR	502
Bmpr2	TGGCAGTGAGGTCACTCAAG	TTGCGTTCATTCTGCATAGC	53	QPCR	178
Acvr2a	AGCAAGGGGAAGATTTGGTT	GGTGCCTCTTTTCTCTGCAC	53	QPCR	168
Acvr2b	CTGTGCGACTCCTTTAAGC	TCTTCACAGCCACAAAGTCG	54	QPCR	174
HPRT	TGCGACCTTGACCATCTTTGG	GCCATCACATTGTAGCCCTCT	56	QPCR	358
ID1	ATCAGGGACCTTCAGTTGGAGC	AGACCCACAGAGCACGTAATTCC	60	QPCR	236
ID2	TCCCAGGGTGTTCTCTTACTTGGACT	GGATCCTTCTGGTATTCACGCTCCA	54	QPCR	200
GDF15	ACGGGAGGTGCAAGTGACCATGTG	TGAGCACCATGGGATTGTTAGC	61	QPCR	158
BMPRII	GCTAAAATTTGGCAGCAAGC	CTTGGGCCCTATGTGTCACT	57	QPCR	224
ENG	CACTAGCCAGGTCTCGAAGG	CTGAGGACCAGAAGCACCTC	57	QPCR	165
SMAD6	AGGAGAAACTCGCTCCAAGTGCAT	AGCTCCCTTCTTACTCCCTGCAAA	54	QPCR	152
SMAD7	TCGGACAGCTCAATTCGGACAACA	AACACCTTGTGTACCAACAGCGTC	54	QPCR	190
ALK1	CCTTGCTGGCCCTGGTGGCCCT	GTGGGCAATGGCTGGTTTG	63	30	607
ALK2	GAAGGGCTCATCACCACCAAT	GAACGGTGGCTTGTAATCCTC	56	26	771
ALK3	GCACATTGCTTTGCCATCATA	CATTTGCCCATCCATACTTCT	60	24	512
ALK6	ACACCACAGGGCTTTACTTAT	AATTGCTGGTTTGCCTTGAGT	56	30	616

Genes	Primer sequences, 5′-3′		Annealing temp, °C	Cycle	Size, bp*
Genes	Forward	Reverse	Annealing temp, °C	Cycle	Size, bp*
Hprt	GCTGGTGAAAAGGACCTCT	CACAGGACTAGAACACCTGC	59	28 or QPCR	280
Alk1	TGCCTGTTCAGATGCCTTCAG	CTTGGGGAGCTTCAGAAGGGG	59	QPCR	502
Bmpr2	TGGCAGTGAGGTCACTCAAG	TTGCGTTCATTCTGCATAGC	53	QPCR	178
Acvr2a	AGCAAGGGGAAGATTTGGTT	GGTGCCTCTTTTCTCTGCAC	53	QPCR	168
Acvr2b	CTGTGCGACTCCTTTAAGC	TCTTCACAGCCACAAAGTCG	54	QPCR	174
HPRT	TGCGACCTTGACCATCTTTGG	GCCATCACATTGTAGCCCTCT	56	QPCR	358
ID1	ATCAGGGACCTTCAGTTGGAGC	AGACCCACAGAGCACGTAATTCC	60	QPCR	236
ID2	TCCCAGGGTGTTCTCTTACTTGGACT	GGATCCTTCTGGTATTCACGCTCCA	54	QPCR	200
GDF15	ACGGGAGGTGCAAGTGACCATGTG	TGAGCACCATGGGATTGTTAGC	61	QPCR	158
BMPRII	GCTAAAATTTGGCAGCAAGC	CTTGGGCCCTATGTGTCACT	57	QPCR	224
ENG	CACTAGCCAGGTCTCGAAGG	CTGAGGACCAGAAGCACCTC	57	QPCR	165
SMAD6	AGGAGAAACTCGCTCCAAGTGCAT	AGCTCCCTTCTTACTCCCTGCAAA	54	QPCR	152
SMAD7	TCGGACAGCTCAATTCGGACAACA	AACACCTTGTGTACCAACAGCGTC	54	QPCR	190
ALK1	CCTTGCTGGCCCTGGTGGCCCT	GTGGGCAATGGCTGGTTTG	63	30	607
ALK2	GAAGGGCTCATCACCACCAAT	GAACGGTGGCTTGTAATCCTC	56	26	771
ALK3	GCACATTGCTTTGCCATCATA	CATTTGCCCATCCATACTTCT	60	24	512
ALK6	ACACCACAGGGCTTTACTTAT	AATTGCTGGTTTGCCTTGAGT	56	30	616

Size of the amplified cDNA.