Effect of IL-27 on HIV replication and HIV transcription
| IL-27 treatment . | HIV replication . | Transcription . |
|---|---|---|
| Experiment 1 | ||
| Absence | (1300 ± 135 (100) | 15.6 ± 0.2 (100) |
| Presence | 478 ± 29 (36) | 5.7 ± 0.2 (39) |
| Experiment 2 | ||
| Absence | 234 ± 30 (100) | 4.96 ± 0.16 (100) |
| Presence | 49 ± 3.0 (21) | 1.03 ± 0.034 (21) |
| IL-27 treatment . | HIV replication . | Transcription . |
|---|---|---|
| Experiment 1 | ||
| Absence | (1300 ± 135 (100) | 15.6 ± 0.2 (100) |
| Presence | 478 ± 29 (36) | 5.7 ± 0.2 (39) |
| Experiment 2 | ||
| Absence | 234 ± 30 (100) | 4.96 ± 0.16 (100) |
| Presence | 49 ± 3.0 (21) | 1.03 ± 0.034 (21) |
X4-infected CD4+ T cells were cultured for 7 days in the absence or presence of 100 ng/mL recombinant IL-27. HIV replication was monitored using p24 antigen capture assay. Data show means ± SD (ng/mL). A total cellular RNA was extracted from the cultured cells as described in “Quantitation of transcribed HIV RNA,” and then the copy number of HIV transcripts was determined by a real-time quantitative RT-PCR. Copy numbers determined from real-time quantitative PCR experiments were adjusted for cell equivalents using CCR5. Data show means ± SD (× 108 copies/106 cells). Values in parentheses show percentage of activity compared with untreated cells.