Effect of VLP treatment on the expression of cytokine genes
| Gene ID . | Gene name . | Fold increase* . | |
|---|---|---|---|
| PBMC . | MDM . | ||
| 24678 | IL27 | 14.0 | 20.0 |
| 6355 | MCP2 | 31.0 | 30.0 |
| 10673 | TNSF13B | 2.6 | 2.5 |
| 3586 | IL10 | NS | 2.3 |
| 8743 | TNSF10 | 6.4 | 111.0 |
| 3600 | IL15 | NS | 4.7 |
| 6373 | CXCL11 | 85.0 | 54.0 |
| 3627 | CXCL10 | 10.0 | 58.0 |
| 6347 | MCP1 | 15.0 | NS |
| 3469 | IFNA | 143.0 | NS |
| 3456 | IFNB | 135.0 | NS |
| Gene ID . | Gene name . | Fold increase* . | |
|---|---|---|---|
| PBMC . | MDM . | ||
| 24678 | IL27 | 14.0 | 20.0 |
| 6355 | MCP2 | 31.0 | 30.0 |
| 10673 | TNSF13B | 2.6 | 2.5 |
| 3586 | IL10 | NS | 2.3 |
| 8743 | TNSF10 | 6.4 | 111.0 |
| 3600 | IL15 | NS | 4.7 |
| 6373 | CXCL11 | 85.0 | 54.0 |
| 3627 | CXCL10 | 10.0 | 58.0 |
| 6347 | MCP1 | 15.0 | NS |
| 3469 | IFNA | 143.0 | NS |
| 3456 | IFNB | 135.0 | NS |
PHA-stimulated PBMCs and MDMs were cultured in the absence or presence of 1 μg/mL VLPs for 36 and 24 hours, respectively. A total RNA was extracted from the cells, and then DNA microarray analysis was performed as described in “Microarray analysis.” Data show genes known to be associated with cytokines which were selected using the DAVID bioinformatics tool.41
NS indicates not significant; TNSF10, TRAIL (tumor necrosis factor–related apoptosis-inducing ligand).
The data show means of fold increases in VLP-treated cells compared with those in untreated cells from 3 independent experiments.