Table 1 Sequences of PCR primers Target... | American Society of Hematology

Table 1

Sequences of PCR primers

Target sequence	Sequence	PCR product length, bp	Source
TF
Forward	5′-CTACTGTTTCAGTGTTCAAGCAGTGA-3′	cDNA, 283	Potgens et al⁴⁸
Reverse	5′-CAGTGCAATATAGCATTTGCAGTAGC-3′	gDNA, 2007	Potgens et al⁴⁸
β-actin
Forward	5′-GAGAAGATGACCCAGATCATGT-3′	cDNA, 542	Funk and FitzGerald⁴⁹
Reverse	5′-GTG TTG GCG TAC AGG TCT TTG-3′	gDNA, 1078	Castelló et al⁵⁰
CD14
Forward	5′-AAAGCACTTCCAGAGCCTGC-3′	cDNA, 422	Current study
Reverse	5′-TCGAGCGTCAGTTCCTTGAGG-3′	gDNA, 512	Current study
GPIbα
Forward	5′-AAGGCCATGACCTCTAACGT-3′	cDNA, 239	Current study
Reverse	5′-GCAGTGGACCATGAGTAGAATAG-3′	gDNA, 239	Current study

Target sequence	Sequence	PCR product length, bp	Source
TF
Forward	5′-CTACTGTTTCAGTGTTCAAGCAGTGA-3′	cDNA, 283	Potgens et al⁴⁸
Reverse	5′-CAGTGCAATATAGCATTTGCAGTAGC-3′	gDNA, 2007	Potgens et al⁴⁸
β-actin
Forward	5′-GAGAAGATGACCCAGATCATGT-3′	cDNA, 542	Funk and FitzGerald⁴⁹
Reverse	5′-GTG TTG GCG TAC AGG TCT TTG-3′	gDNA, 1078	Castelló et al⁵⁰
CD14
Forward	5′-AAAGCACTTCCAGAGCCTGC-3′	cDNA, 422	Current study
Reverse	5′-TCGAGCGTCAGTTCCTTGAGG-3′	gDNA, 512	Current study
GPIbα
Forward	5′-AAGGCCATGACCTCTAACGT-3′	cDNA, 239	Current study
Reverse	5′-GCAGTGGACCATGAGTAGAATAG-3′	gDNA, 239	Current study