Trisomy 8 CD34 cells are primarily annexin+, and cell-cycle analysis of these demonstrated no DNA degradation
| . | Annexin−, % . | Annexin+, % . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Trisomy 8 . | Subgenomic DNA . | G1 . | S . | G2 . | Trisomy 8 . | Subgenomic DNA . | G1 . | S . | G2 . | Positive TUNEL assay . |
| 1 | 15 | 1 | 80 | 15 | 4 | 85 | 1 | 97 | 2 | 0 | 5 |
| 8 | 17 | 0 | 88 | 2 | 10 | 83 | 1 | 97 | 1 | 1 | 10 |
| 21 | 10 | 1 | 83 | 15 | 2 | 58 | 1 | 68 | 1 | 0 | ND |
| 7 | 4 | 1 | 98 | 1 | 0 | 98 | 2 | 98 | 0 | 0 | 3 |
| 13 | 3 | 0 | 90 | 10 | 0 | 70 | 3 | 97 | 0 | 0 | 7 |
| 3 | 2 | ND | ND | ND | ND | 43 | ND | ND | ND | ND | 20 |
| 23 | 13 | ND | ND | ND | ND | 87 | ND | ND | ND | ND | ND |
| 10 | 3 | ND | ND | ND | ND | 85 | ND | ND | ND | ND | ND |
| 34 | 2 | ND | ND | ND | ND | 97 | ND | ND | ND | ND | 4 |
| Mean healthy control* | 0 | 0 | 98 | 1 | 1 | 0 | 56 | 40 | 0 | 0 | 68 |
| . | Annexin−, % . | Annexin+, % . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Trisomy 8 . | Subgenomic DNA . | G1 . | S . | G2 . | Trisomy 8 . | Subgenomic DNA . | G1 . | S . | G2 . | Positive TUNEL assay . |
| 1 | 15 | 1 | 80 | 15 | 4 | 85 | 1 | 97 | 2 | 0 | 5 |
| 8 | 17 | 0 | 88 | 2 | 10 | 83 | 1 | 97 | 1 | 1 | 10 |
| 21 | 10 | 1 | 83 | 15 | 2 | 58 | 1 | 68 | 1 | 0 | ND |
| 7 | 4 | 1 | 98 | 1 | 0 | 98 | 2 | 98 | 0 | 0 | 3 |
| 13 | 3 | 0 | 90 | 10 | 0 | 70 | 3 | 97 | 0 | 0 | 7 |
| 3 | 2 | ND | ND | ND | ND | 43 | ND | ND | ND | ND | 20 |
| 23 | 13 | ND | ND | ND | ND | 87 | ND | ND | ND | ND | ND |
| 10 | 3 | ND | ND | ND | ND | 85 | ND | ND | ND | ND | ND |
| 34 | 2 | ND | ND | ND | ND | 97 | ND | ND | ND | ND | 4 |
| Mean healthy control* | 0 | 0 | 98 | 1 | 1 | 0 | 56 | 40 | 0 | 0 | 68 |
CD34+, annexin+ BMMNCs obtained from patients with trisomy 8 and purified by flow cytometry were analyzed by FISH. Samples from these patients, which were subjected to cell-cycle analysis or TUNEL assay as described in “Patients, methods, and materials,” showed little DNA degradation. Results were compared with an apoptotic control (normal BMMNCs prompted to undergo apoptosis by coculture with IFNγ [1000 U/mL] and Fas agonist).
ND indicates not done.
* N = 5.