Table 1

BrdU pulse-labeled cells in bone marrow and spleen

FractionPhenotype% of BrdU+ cells in fraction* (day of peak labeling)
Bone marrow   
    A-D CD45R+IgMIgD 50.0 (1 d) 
    E IgM+IgD 47.0 (2 d) 
    NF/T1 IgMhiIgDloCD21lo 38.2 (2 d) 
    F-II IgDhiIgMhiCD21int 19.5 (3 d) 
    F-I IgDhiIgMlo 7.5 (3 d) 
Spleen   
    NF/T1 IgMhiIgDlo/−CD21lo 18.7 (2 d) 
    F-II IgDhiIgMhiCD21int 6.2 (3 d) 
    F-I IgDhiIgMlo 3.5 (5 d) 
FractionPhenotype% of BrdU+ cells in fraction* (day of peak labeling)
Bone marrow   
    A-D CD45R+IgMIgD 50.0 (1 d) 
    E IgM+IgD 47.0 (2 d) 
    NF/T1 IgMhiIgDloCD21lo 38.2 (2 d) 
    F-II IgDhiIgMhiCD21int 19.5 (3 d) 
    F-I IgDhiIgMlo 7.5 (3 d) 
Spleen   
    NF/T1 IgMhiIgDlo/−CD21lo 18.7 (2 d) 
    F-II IgDhiIgMhiCD21int 6.2 (3 d) 
    F-I IgDhiIgMlo 3.5 (5 d) 
*

Figure 2A presents the absolute numbers in fractions NF/T1, F-II, and F-I. Number of mice examined as in legend for Figure 2.

Most rapid influx on day 2. No statistical difference between days 2 and 3 (Figure 2A).

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