Characterization of bone marrow and splenic hematopoiesis in gp130Y757F/Y757F mice lacking either IL-6 or IL-11Rα
| Parameter . | +/+ . | F/F . | F/F:IL-6−/− . | F/F:IL-11R−/− . |
|---|---|---|---|---|
| Bone marrow per femur* | ||||
| Cellularity, × 107 | 4.15 ± 0.89 | 4.06 ± 0.53 | 3.98 ± 0.69 | 3.71 ± 1.46 |
| Blasts, % | 6 ± 3 | 8 ± 1 | 5 ± 3 | 8 ± 6 |
| Promyelocytes/myelocytes, % | 6 ± 2 | 13 ± 5‡ | 12 ± 3‡ | 7 ± 1 |
| Metamyelocytes/neutrophils, % | 30 ± 8 | 43 ± 5‡ | 32 ± 8 | 35 ± 9 |
| Lymphocytes, % | 35 ± 9 | 19 ± 5‡ | 31 ± 9 | 34 ± 11 |
| Monocytes, % | 4 ± 2 | 3 ± 2 | 3 ± 1 | 3 ± 2 |
| Eosinophils, % | 3 ± 1 | 3 ± 2 | 2 ± 1 | 2 ± 2 |
| Nucleated RBCs, % | 16 ± 4 | 10 ± 5 | 15 ± 2 | 11 ± 4 |
| Megakaryocytes, × 104 | 1.54 ± 0.35 | 2.83 ± 0.64§ | 1.59 ± 0.37 | 1.28 ± 0.35 |
| Total CFC† | 27 ± 8 | 40 ± 9‡ | 26 ± 9 | 30 ± 12 |
| Spleen* | ||||
| Mass, mg | 87 ± 8 | 222 ± 36§ | 132 ± 22‡ | 201 ± 26§ |
| Cellularity, × 108 | 1.35 ± 0.54 | 3.82 ± 1.57§ | 1.52 ± 0.60 | 2.90 ± 0.98‡ |
| Blasts, % | 1 ± 1 | 1 ± 1 | 0 ± 0 | 0 ± 0 |
| Promyelocytes/myelocytes, % | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Metamyelocytes/neutrophils, % | 3 ± 2 | 8 ± 5§ | 6 ± 2 | 10 ± 4§ |
| Lymphocytes, % | 89 ± 3 | 76 ± 8‡ | 89 ± 3 | 80 ± 8 |
| Monocytes, % | 1 ± 1 | 3 ± 2 | 1 ± 1 | 4 ± 2 |
| Eosinophils, % | 0 ± 1 | 0 ± 0 | 1 ± 1 | 0 ± 1 |
| Nucleated RBCs, % | 5 ± 3 | 14 ± 5‡ | 3 ± 2 | 7 ± 3§ |
| Megakaryocytes, × 103 | 1.66 ± 0.31 | 15.94 ± 3.69§ | 4.19 ± 1.10§ | 18.06 ± 7.82§ |
| Total CFC† | 3 ± 2 | 13 ± 8‡ | 3 ± 1 | 7 ± 2‡ |
| Parameter . | +/+ . | F/F . | F/F:IL-6−/− . | F/F:IL-11R−/− . |
|---|---|---|---|---|
| Bone marrow per femur* | ||||
| Cellularity, × 107 | 4.15 ± 0.89 | 4.06 ± 0.53 | 3.98 ± 0.69 | 3.71 ± 1.46 |
| Blasts, % | 6 ± 3 | 8 ± 1 | 5 ± 3 | 8 ± 6 |
| Promyelocytes/myelocytes, % | 6 ± 2 | 13 ± 5‡ | 12 ± 3‡ | 7 ± 1 |
| Metamyelocytes/neutrophils, % | 30 ± 8 | 43 ± 5‡ | 32 ± 8 | 35 ± 9 |
| Lymphocytes, % | 35 ± 9 | 19 ± 5‡ | 31 ± 9 | 34 ± 11 |
| Monocytes, % | 4 ± 2 | 3 ± 2 | 3 ± 1 | 3 ± 2 |
| Eosinophils, % | 3 ± 1 | 3 ± 2 | 2 ± 1 | 2 ± 2 |
| Nucleated RBCs, % | 16 ± 4 | 10 ± 5 | 15 ± 2 | 11 ± 4 |
| Megakaryocytes, × 104 | 1.54 ± 0.35 | 2.83 ± 0.64§ | 1.59 ± 0.37 | 1.28 ± 0.35 |
| Total CFC† | 27 ± 8 | 40 ± 9‡ | 26 ± 9 | 30 ± 12 |
| Spleen* | ||||
| Mass, mg | 87 ± 8 | 222 ± 36§ | 132 ± 22‡ | 201 ± 26§ |
| Cellularity, × 108 | 1.35 ± 0.54 | 3.82 ± 1.57§ | 1.52 ± 0.60 | 2.90 ± 0.98‡ |
| Blasts, % | 1 ± 1 | 1 ± 1 | 0 ± 0 | 0 ± 0 |
| Promyelocytes/myelocytes, % | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Metamyelocytes/neutrophils, % | 3 ± 2 | 8 ± 5§ | 6 ± 2 | 10 ± 4§ |
| Lymphocytes, % | 89 ± 3 | 76 ± 8‡ | 89 ± 3 | 80 ± 8 |
| Monocytes, % | 1 ± 1 | 3 ± 2 | 1 ± 1 | 4 ± 2 |
| Eosinophils, % | 0 ± 1 | 0 ± 0 | 1 ± 1 | 0 ± 1 |
| Nucleated RBCs, % | 5 ± 3 | 14 ± 5‡ | 3 ± 2 | 7 ± 3§ |
| Megakaryocytes, × 103 | 1.66 ± 0.31 | 15.94 ± 3.69§ | 4.19 ± 1.10§ | 18.06 ± 7.82§ |
| Total CFC† | 3 ± 2 | 13 ± 8‡ | 3 ± 1 | 7 ± 2‡ |
*Values represent the mean ± SD from at least 5 mice for each genotype.
†Number of colony-forming cells (CFCs) in semisolid agar cultures containing 2.5 × 104 bone marrow cells or 2.5 × 105 spleen cells. Cells were stimulated with IL-3 and SCF.
‡P < .05 and
§P < .001 versus data from gp130+/+ mice. No other statistically significant differences were observed between genotypes.