DLI-derived T-cell expansion and production of IFN-γ in MHC-matched, mHAg-mismatched chimeras is not influenced by the amount of residual host APCs
| Chimera and time of DLI . | Source of DLI . | Day 5 . | Day 12 . | Day 29 . | |||
|---|---|---|---|---|---|---|---|
| CD4+ . | CD8+ . | CD4+ . | CD8+ . | CD4+ . | CD8+ . | ||
| T-cell expansion, 8 wk, cells/spleen × 105 | |||||||
| B10.D2+BALB/c→BALB/c | Naive | 2.0 ± 0.02 | 1.1 ± 0.01 | 2.5 ± 0.1 | 1.7 ± 0.07 | 1.5 ± 0.04 | 1.2 ± 0.02 |
| B10.D2→BALB/c | Naive | 1.7 ± 0.03 | 1.0 ± 0.01 | 2.9 ± 0.15 | 2.4 ± 0.11 | 1.4 ± 0.02 | 1.5 ± 0.04 |
| B10.D2+BALB/c→BALB/c | Presensitized | 2.3 ± 0.02 | 1.3 ± 0.02 | 2.0 ± 0.08 | 1.4 ± 0.04 | 1.9 ± 0.01 | 1.5 ± 0.06 |
| B10.D2→BALB/c | Presensitized | 7.1 ± 0.07 | 3.8 ± 0.06 | 5.9 ± 0.17* | 42.5 ± 1.3* | 3.7 ± 0.18 | 7.6 ± 0.35* |
| IFN-γ production, 8 wk, cells/spleen × 104 | |||||||
| B10.D2+BALB/c→BALB/c | Naive | 1.78 ± 0.3 | 0.31 ± 0.23 | 1.19 ± 0.32 | 1.79 ± 0.88 | 0.81 ± 0.28 | 0.48 ± 0.17 |
| B10.D2→BALB/c | Naive | 1.63 ± 0.1 | 0.26 ± 0.08 | 2.23 ± 1.31 | 1.99 ± 0.95 | 0.89 ± 0.22 | 1.15 ± 0.71 |
| Chimera and time of DLI . | Source of DLI . | Day 5 . | Day 12 . | Day 29 . | |||
|---|---|---|---|---|---|---|---|
| CD4+ . | CD8+ . | CD4+ . | CD8+ . | CD4+ . | CD8+ . | ||
| T-cell expansion, 8 wk, cells/spleen × 105 | |||||||
| B10.D2+BALB/c→BALB/c | Naive | 2.0 ± 0.02 | 1.1 ± 0.01 | 2.5 ± 0.1 | 1.7 ± 0.07 | 1.5 ± 0.04 | 1.2 ± 0.02 |
| B10.D2→BALB/c | Naive | 1.7 ± 0.03 | 1.0 ± 0.01 | 2.9 ± 0.15 | 2.4 ± 0.11 | 1.4 ± 0.02 | 1.5 ± 0.04 |
| B10.D2+BALB/c→BALB/c | Presensitized | 2.3 ± 0.02 | 1.3 ± 0.02 | 2.0 ± 0.08 | 1.4 ± 0.04 | 1.9 ± 0.01 | 1.5 ± 0.06 |
| B10.D2→BALB/c | Presensitized | 7.1 ± 0.07 | 3.8 ± 0.06 | 5.9 ± 0.17* | 42.5 ± 1.3* | 3.7 ± 0.18 | 7.6 ± 0.35* |
| IFN-γ production, 8 wk, cells/spleen × 104 | |||||||
| B10.D2+BALB/c→BALB/c | Naive | 1.78 ± 0.3 | 0.31 ± 0.23 | 1.19 ± 0.32 | 1.79 ± 0.88 | 0.81 ± 0.28 | 0.48 ± 0.17 |
| B10.D2→BALB/c | Naive | 1.63 ± 0.1 | 0.26 ± 0.08 | 2.23 ± 1.31 | 1.99 ± 0.95 | 0.89 ± 0.22 | 1.15 ± 0.71 |
The absolute number of DLI-derived Thy 1.1+ T cells was calculated on days +5, +12, and +29 after DLI (2 × 107 B10.D2-Thy1.1 splenocytes) by multiplying the total number of viable cells by the percentages of gated CD4+ or CD8+ T cells coexpressing CD90.1 marker, respectively (n=3–4 chimeras/time point and group).
P<.05; between the B10.D2→BALB/c group that received presensitized DLI versus other groups.