Effect of a single intraperitoneal injection of denileukin diftitox on Treg cells in various lymphoid compartments in C57BL/6 versus BALB/c mice
| . | Naive . | Day 1 . | Day 3 . | Day 5 . | Day 10 . |
|---|---|---|---|---|---|
| C57BL/6 mice | |||||
| Spleen | 9.9 ± 0.5 | 7.6 ± 0.5* | 7.3 ± 0.4* | 8.3 ± 0.6* | 8.2 ± 1.0* |
| Blood | 6.1 ± 0.4 | 4.8 ± 0.3* | 5.6 ± 0.5 | 5.6 ± 0.8 | 5.6 ± 0.2 |
| Bone marrow | 10.2 ± 0.6 | 7.7 ± 1.4* | 7.8 ± 0.7 | 7.6 ± 0.2* | 6.8 ± 1.9* |
| BALB/c mice | |||||
| Spleen | 8.3 ± 1.1 | 9.0 ± 0.3 | 8.5 ± 0.9 | 8.3 ± 0.9 | 8.2 ± 0.4 |
| Blood | 5.9 ± 0.3 | 5.1 ± 0.3* | 5.4 ± 0.2 | 5.6 ± 0.4 | 5.6 ± 0.5 |
| Bone marrow | 10.9 ± 1.8 | 10.3 ± 2.0 | 6.4 ± 1.9 | 8.2 ± 3.3 | 11.1 ± 4.5 |
| . | Naive . | Day 1 . | Day 3 . | Day 5 . | Day 10 . |
|---|---|---|---|---|---|
| C57BL/6 mice | |||||
| Spleen | 9.9 ± 0.5 | 7.6 ± 0.5* | 7.3 ± 0.4* | 8.3 ± 0.6* | 8.2 ± 1.0* |
| Blood | 6.1 ± 0.4 | 4.8 ± 0.3* | 5.6 ± 0.5 | 5.6 ± 0.8 | 5.6 ± 0.2 |
| Bone marrow | 10.2 ± 0.6 | 7.7 ± 1.4* | 7.8 ± 0.7 | 7.6 ± 0.2* | 6.8 ± 1.9* |
| BALB/c mice | |||||
| Spleen | 8.3 ± 1.1 | 9.0 ± 0.3 | 8.5 ± 0.9 | 8.3 ± 0.9 | 8.2 ± 0.4 |
| Blood | 5.9 ± 0.3 | 5.1 ± 0.3* | 5.4 ± 0.2 | 5.6 ± 0.4 | 5.6 ± 0.5 |
| Bone marrow | 10.9 ± 1.8 | 10.3 ± 2.0 | 6.4 ± 1.9 | 8.2 ± 3.3 | 11.1 ± 4.5 |
C57BL/6 or BALB/c mice were either untreated (naive) or treated with 0.75 μg denileukin diftitox intraperitoneally. Mice were killed on the indicated days after denileukin diftitox injection. Cells from individual mice were stained for CD4+CD25+Foxp3+ Treg cells; value shown is mean percentage CD25+Foxp3+ of total CD4+ cells (n = 4).
Statistical evaluation was performed by one-way ANOVA using GraphPad Prism.
P < .05 versus naive.