Predicted and observed ratio of mutant to WT expression in double HS deletion mice
| Double HS mutation . | Predicted deficit . | Predicted ratio . | Observed ratio, bulk RNA . | Observed ratio, single cells . |
|---|---|---|---|---|
| Δ1,4 | 0.41 | 0.59 | 0.60 ± 0.03 | 0.64 ± 0.07 |
| Δ1-2 | 0.63 | 0.37 | 0.39 ± 0.01 | 0.41 ± 0.07 |
| Δ2-3 | 0.70 | 0.30 | 0.31 ± 0.02 | 0.32 ± 0.09 |
| Double HS mutation . | Predicted deficit . | Predicted ratio . | Observed ratio, bulk RNA . | Observed ratio, single cells . |
|---|---|---|---|---|
| Δ1,4 | 0.41 | 0.59 | 0.60 ± 0.03 | 0.64 ± 0.07 |
| Δ1-2 | 0.63 | 0.37 | 0.39 ± 0.01 | 0.41 ± 0.07 |
| Δ2-3 | 0.70 | 0.30 | 0.31 ± 0.02 | 0.32 ± 0.09 |
The predicted deficit is the sum of the deficits of the two individual HSs deleted (from Table 1). The predicted ratio is calculated from the predicted deficit. The observed ratio (bulk RNA) is the experimentally determined average ratio of expression of the mutant allele to the WT allele from 6 mice, followed by the SD. The observed ratio (single cells) is the ratio of expression of the mutant allele to the WT allele, followed by the SD.