Sequences of primers and TaqMan probes used in quantitative real-time RT-PCR
| PCR primer . | Sequence . |
|---|---|
| γ-globin | |
| Forward | AATGTGGAAGATGCTGGAGGAGAA |
| Reverse | CTTCTTGCCATGTGCCTTGACTT |
| Probe | TexRd-XN-CCCTGGGAAGGCTCCTGGTTGTCTACCC-BHQ2 |
| β-globin | |
| Forward | CCTGAGGAGAAGTCTGCCGTTAC |
| Reverse | TAGACCACCAGCAGCCTGCC |
| Probe | TexRd-XN-ACCACCAACTTCATCCACGTTCACCTTGCC-BHQ2 |
| PCR primer . | Sequence . |
|---|---|
| γ-globin | |
| Forward | AATGTGGAAGATGCTGGAGGAGAA |
| Reverse | CTTCTTGCCATGTGCCTTGACTT |
| Probe | TexRd-XN-CCCTGGGAAGGCTCCTGGTTGTCTACCC-BHQ2 |
| β-globin | |
| Forward | CCTGAGGAGAAGTCTGCCGTTAC |
| Reverse | TAGACCACCAGCAGCCTGCC |
| Probe | TexRd-XN-ACCACCAACTTCATCCACGTTCACCTTGCC-BHQ2 |
TexRd-XN indicates Texas red-XN; BHQ2, black hole quencher 2.