Sequences of primers and TaqMan probes used in quantitative real-time PCR for the ChIP assay
| PCR primer . | Sequence . |
|---|---|
| ϵ-globin | |
| Forward | AGAGAGGCAGCAGCACATATCTG |
| Reverse | CATCTTGCTCCACAGGCTAGTGA |
| Probe | FAM-AGCTGCAATCACTAGCAAGCTCTCAGGCC-BHQ1 |
| γ-globin | |
| Forward | GGCTGGCTAGGGATGAAGAATAAA |
| Reverse | TGGCGTCTGGACTAGGAGCTTA |
| Probe | TexRd-XN-CCTTCAGCAGTTCCACACACTCGCTTCTGG-BHQ2 |
| β-globin | |
| Forward | AGGGAGGGCTGAGGGTTTGA |
| Reverse | CAGGGTGAGGTCTAAGTGATGACA |
| Probe | FAM-TCCAACTCCTAAGCCAGTGCCAGAAGAGCC-BHQ1 |
| α-actin | |
| Forward | GTCTCCCTGTCCTTGCAGAAACTA |
| Reverse | CCCACGATGGACGGGAACAC |
| Probe | FAM-ACAATGTGCGACGAAGACGAGACCACCG-BHQ1 |
| β-actin | |
| Forward | CCCTGGCGGCCTAAGGACTC |
| Reverse | CACATGCCGGAGCCGTTGTC |
| Probe | HEX-CGACGAGCGCGGCGATATCATCATCCATG-BHQ2 |
| PCR primer . | Sequence . |
|---|---|
| ϵ-globin | |
| Forward | AGAGAGGCAGCAGCACATATCTG |
| Reverse | CATCTTGCTCCACAGGCTAGTGA |
| Probe | FAM-AGCTGCAATCACTAGCAAGCTCTCAGGCC-BHQ1 |
| γ-globin | |
| Forward | GGCTGGCTAGGGATGAAGAATAAA |
| Reverse | TGGCGTCTGGACTAGGAGCTTA |
| Probe | TexRd-XN-CCTTCAGCAGTTCCACACACTCGCTTCTGG-BHQ2 |
| β-globin | |
| Forward | AGGGAGGGCTGAGGGTTTGA |
| Reverse | CAGGGTGAGGTCTAAGTGATGACA |
| Probe | FAM-TCCAACTCCTAAGCCAGTGCCAGAAGAGCC-BHQ1 |
| α-actin | |
| Forward | GTCTCCCTGTCCTTGCAGAAACTA |
| Reverse | CCCACGATGGACGGGAACAC |
| Probe | FAM-ACAATGTGCGACGAAGACGAGACCACCG-BHQ1 |
| β-actin | |
| Forward | CCCTGGCGGCCTAAGGACTC |
| Reverse | CACATGCCGGAGCCGTTGTC |
| Probe | HEX-CGACGAGCGCGGCGATATCATCATCCATG-BHQ2 |
FAM indicates 6-carboxyfluorescein; BHQ1, black hole quencher 1; TexRd-XN, Texas red-X NHS ester; BHQ2, black hole quencher 2; HEX, hexachlorofluorescein.