Analysis of data from 19 HIV+ patients used for PBMC and CD34+ cell proviral analysis
| Patient no. . | CD4, count/μL . | Sex . | HGB, g/dL . | PBMC proviral, per 104 cells . | CD34+ proviral, per 104 cells . |
|---|---|---|---|---|---|
| 1 | 153 | F | 11.7 | 34.8 | 0.0 |
| 2 | 94 | F | 13.9 | 7.3 | 0.0 |
| 3*† | 50 | M | 9.8 | 17.32 | 1262 |
| 4* | 104 | M | 15.6 | 77.0 | 5300 |
| 5* | 94 | M | 11.3 | 106 | 1034 |
| 6*† | 13 | F | 9.8 | 3.74 | 3800 |
| 7 | 44 | F | 11 | 78.0 | 0.0 |
| 8 | 226 | F | 11.9 | 47.6 | 145.4 |
| 9†‡ | 90 | F | 9.1 | 45 | 195.8 |
| 10 | 124 | M | 14.5 | 68.4 | 0.0 |
| 11 | 75 | F | 11.7 | 5.14 | 0.0 |
| 12 | 143 | M | 14.4 | 38.6 | 0.0 |
| 13*† | 113 | M | 7.7 | 36.8 | 354 |
| 14† | 10 | F | 9.6 | 146.6 | 0.0 |
| 15†‡ | 5 | M | 9 | 38.6 | 238 |
| 16* | 53 | M | 15.6 | 116.2 | 742 |
| 17*† | 54 | F | 10 | 66.8 | 2080 |
| 18*† | 71 | F | 8.3 | 101.2 | 4500 |
| 19*† | 186 | F | 4.8 | 142 | 2200 |
| Patient no. . | CD4, count/μL . | Sex . | HGB, g/dL . | PBMC proviral, per 104 cells . | CD34+ proviral, per 104 cells . |
|---|---|---|---|---|---|
| 1 | 153 | F | 11.7 | 34.8 | 0.0 |
| 2 | 94 | F | 13.9 | 7.3 | 0.0 |
| 3*† | 50 | M | 9.8 | 17.32 | 1262 |
| 4* | 104 | M | 15.6 | 77.0 | 5300 |
| 5* | 94 | M | 11.3 | 106 | 1034 |
| 6*† | 13 | F | 9.8 | 3.74 | 3800 |
| 7 | 44 | F | 11 | 78.0 | 0.0 |
| 8 | 226 | F | 11.9 | 47.6 | 145.4 |
| 9†‡ | 90 | F | 9.1 | 45 | 195.8 |
| 10 | 124 | M | 14.5 | 68.4 | 0.0 |
| 11 | 75 | F | 11.7 | 5.14 | 0.0 |
| 12 | 143 | M | 14.4 | 38.6 | 0.0 |
| 13*† | 113 | M | 7.7 | 36.8 | 354 |
| 14† | 10 | F | 9.6 | 146.6 | 0.0 |
| 15†‡ | 5 | M | 9 | 38.6 | 238 |
| 16* | 53 | M | 15.6 | 116.2 | 742 |
| 17*† | 54 | F | 10 | 66.8 | 2080 |
| 18*† | 71 | F | 8.3 | 101.2 | 4500 |
| 19*† | 186 | F | 4.8 | 142 | 2200 |
Samples were collected from HIV-positive patients beginning the national HAART regimen at the Infectious Disease Care Clinic in Gaborone, Botswana. All patients were antiretroviral drug–naive. CD34+ cells were isolated using a magnetic bead positive selection (Invitrogen). Patients with a CD34+ cell proviral load that was significantly higher than their respective PBMC proviral load are indicated *(t test, P < .005, ‡P < .05). Eight of 9 anemic patients (nos. 3, 6, 9, 13, 15, and 17-19) had positive CD34+ cell populations, a significantly higher portion than the 3 of 10 nonanemic patients (nos. 4, 5, and 16; Fisher exact test, P < .05).
Patients who had anemia (HGB ≤ 10 g/dL).