Number of clonal expansions with intraclonal diversity grouped by donor and B-cell subset
| Donors (n) . | B-cell subset . | No. of clonal expansions . | Individuals with clonal expansions . | Somatically mutated IGH VDJ gene clones* . | Average no. of clones per individual (95% CI) . |
|---|---|---|---|---|---|
| Healthy individuals (7) | Naive | 0 | 0/7 | n.a. | n.d. |
| CD5+ | 0 | 0/7 | n.a. | n.d. | |
| CSM | 1 | 1/7 | 1 | 0.14 (0.00-0.43) | |
| NCSM | 25 | 6/7 | 25 | 3.57 (1.29-6.71) | |
| HCV+ patients (22) | Naïve | 5 | 3/22 | 5 | n.d. |
| CD5+ | 2 | 2/22 | 2 | n.d. | |
| CSM | 35 | 13/22 | 35 | 1.59 (0.68-2.73) | |
| NCSM | 122 | 17/22 | 119 | 5.55 (2.82-8.91) |
| Donors (n) . | B-cell subset . | No. of clonal expansions . | Individuals with clonal expansions . | Somatically mutated IGH VDJ gene clones* . | Average no. of clones per individual (95% CI) . |
|---|---|---|---|---|---|
| Healthy individuals (7) | Naive | 0 | 0/7 | n.a. | n.d. |
| CD5+ | 0 | 0/7 | n.a. | n.d. | |
| CSM | 1 | 1/7 | 1 | 0.14 (0.00-0.43) | |
| NCSM | 25 | 6/7 | 25 | 3.57 (1.29-6.71) | |
| HCV+ patients (22) | Naïve | 5 | 3/22 | 5 | n.d. |
| CD5+ | 2 | 2/22 | 2 | n.d. | |
| CSM | 35 | 13/22 | 35 | 1.59 (0.68-2.73) | |
| NCSM | 122 | 17/22 | 119 | 5.55 (2.82-8.91) |
Considered are clonal expansions with at least 5 distinct members in each aliquot of a B-cell subset (further details are given in supplemental Table 5).
n.a., not applicable; n.d., not determined.
We defined a clonal rearrangement as mutated when the mutation frequency was ≥1%.