List of secondary mutations occurring in ruxolitinib-selected TS1 subclones derived from 6 different GF-independent clones
| TS1 clones . | Primary mutation . | Secondary mutation . | Frequency . | ||
|---|---|---|---|---|---|
| JAK1 . | JAK3 . | JAK1 . | JAK3 . | ||
| A3 | Y652H | — | — | Y824D | 8/9 |
| — | — | 1/9 | |||
| A4 | Y652H | — | — | Y904C | 2/9 |
| — | Y904H | 3/9 | |||
| F958C | — | 1/9 | |||
| — | — | 3/9 | |||
| A5 | Y652H | — | — | L586S | 3/11 |
| — | F666L | 1/11 | |||
| — | R840H | 1/11 | |||
| — | Y904C | 5/11 | |||
| L910P | — | 1/11 | |||
| A7 | Y652H | — | Y652H | — | 1/10 |
| F958S | — | 8/10 | |||
| F958S | R840C | 1/10 | |||
| T16 | Y652H | — | — | R840H | 1/9 |
| Y652H | — | 1/9 | |||
| S1042I | — | 2/9 | |||
| — | — | 5/9 | |||
| T18 | Y652H | — | — | Y904C | 1/5 |
| Y652H | — | 2/5 | |||
| F958V | — | 1/5 | |||
| S1042I | — | 1/5 | |||
| TS1 clones . | Primary mutation . | Secondary mutation . | Frequency . | ||
|---|---|---|---|---|---|
| JAK1 . | JAK3 . | JAK1 . | JAK3 . | ||
| A3 | Y652H | — | — | Y824D | 8/9 |
| — | — | 1/9 | |||
| A4 | Y652H | — | — | Y904C | 2/9 |
| — | Y904H | 3/9 | |||
| F958C | — | 1/9 | |||
| — | — | 3/9 | |||
| A5 | Y652H | — | — | L586S | 3/11 |
| — | F666L | 1/11 | |||
| — | R840H | 1/11 | |||
| — | Y904C | 5/11 | |||
| L910P | — | 1/11 | |||
| A7 | Y652H | — | Y652H | — | 1/10 |
| F958S | — | 8/10 | |||
| F958S | R840C | 1/10 | |||
| T16 | Y652H | — | — | R840H | 1/9 |
| Y652H | — | 1/9 | |||
| S1042I | — | 2/9 | |||
| — | — | 5/9 | |||
| T18 | Y652H | — | — | Y904C | 1/5 |
| Y652H | — | 2/5 | |||
| F958V | — | 1/5 | |||
| S1042I | — | 1/5 | |||
The GF-independent TS1 clones with their corresponding primary JAK1 mutation and the secondary mutations identified in their ruxolitinib-selected subclones. For each combination, relative frequency is indicated.