KIR-KIRL repertoire constellations of donors and patients included in this study
| Donor . | KIRL . | Donor education of inhibitory KIRs . | Educated KIRs specific for the missing KIRL in the target . | ||||
|---|---|---|---|---|---|---|---|
| Educated . | Uneducated . | P3B Bw6/Bw6 C1/C1 . | P23T ABw4/ABw4 Bw4/Bw4 C2/C1 . | P31G A3/A3 Bw4/Bw6 C2/C2 . | Nalm-16 Bw4 C1* . | ||
| SNK9A | Bw6/Bw6 C1/C1 | 2DL2, 2DL3 | 2DL1, 3DL1, 3DL2 | — | N/A | 2DL2, 2DL3 | — |
| SNK10P | Bw6/Bw6 C1/C1 | 2DL2, 2DL3 | 2DL1, 3DL1, 3DL2 | — | N/A | 2DL2, 2DL3 | — |
| SNK21BC | A3 Bw6/Bw6 C1/C1 | 2DL2, 2DL3, 3DL2 | 2DL1, 3DL1 | 3DL2 | N/A | 2DL2, 2DL3 | 3DL2 |
| SNK13B | ABw4 Bw4/Bw4 C2/C2 | 2DL1† | 2DL2, 2DL3, 3DL2 | 2DL1† | N/A | — | 2DL1 |
| SNK14B | ABw4 Bw4/Bw4 C2/C2 | 2DL1, 3DL1 | 2DL2, 2DL3, 3DL2 | 2DL1, 3DL1 | N/A | — | 2DL1 |
| SNK15B | Bw4/Bw4 C2/C2 | 2DL1, 3DL1 | 2DL2, 2DL3, 3DL2 | 2DL1, 3DL1 | N/A | — | 2DL1 |
| SNK20B | A3 Bw4/Bw4 C2/C2 | 2DL1, 3DL1, 3DL2 | 2DL2, 2DL3 | 2DL1, 3DL1, 3DL2 | N/A | — | 2DL1, 3DL2 |
| SSC21D | Bw4/Bw4 C1/C2 | 2DL1, 2DL3, 3DL1‡§ | 3DL2 | 2DL1, 3DL1 | — | N/A | N/A |
| SSC18U | ABw4 Bw4/Bw4 C2/C1 | 2DL1, 2DL3, 3DL1‡ | 3DL2 | 2DL1, 3DL1 | N/A | N/A | N/A |
| Donor . | KIRL . | Donor education of inhibitory KIRs . | Educated KIRs specific for the missing KIRL in the target . | ||||
|---|---|---|---|---|---|---|---|
| Educated . | Uneducated . | P3B Bw6/Bw6 C1/C1 . | P23T ABw4/ABw4 Bw4/Bw4 C2/C1 . | P31G A3/A3 Bw4/Bw6 C2/C2 . | Nalm-16 Bw4 C1* . | ||
| SNK9A | Bw6/Bw6 C1/C1 | 2DL2, 2DL3 | 2DL1, 3DL1, 3DL2 | — | N/A | 2DL2, 2DL3 | — |
| SNK10P | Bw6/Bw6 C1/C1 | 2DL2, 2DL3 | 2DL1, 3DL1, 3DL2 | — | N/A | 2DL2, 2DL3 | — |
| SNK21BC | A3 Bw6/Bw6 C1/C1 | 2DL2, 2DL3, 3DL2 | 2DL1, 3DL1 | 3DL2 | N/A | 2DL2, 2DL3 | 3DL2 |
| SNK13B | ABw4 Bw4/Bw4 C2/C2 | 2DL1† | 2DL2, 2DL3, 3DL2 | 2DL1† | N/A | — | 2DL1 |
| SNK14B | ABw4 Bw4/Bw4 C2/C2 | 2DL1, 3DL1 | 2DL2, 2DL3, 3DL2 | 2DL1, 3DL1 | N/A | — | 2DL1 |
| SNK15B | Bw4/Bw4 C2/C2 | 2DL1, 3DL1 | 2DL2, 2DL3, 3DL2 | 2DL1, 3DL1 | N/A | — | 2DL1 |
| SNK20B | A3 Bw4/Bw4 C2/C2 | 2DL1, 3DL1, 3DL2 | 2DL2, 2DL3 | 2DL1, 3DL1, 3DL2 | N/A | — | 2DL1, 3DL2 |
| SSC21D | Bw4/Bw4 C1/C2 | 2DL1, 2DL3, 3DL1‡§ | 3DL2 | 2DL1, 3DL1 | — | N/A | N/A |
| SSC18U | ABw4 Bw4/Bw4 C2/C1 | 2DL1, 2DL3, 3DL1‡ | 3DL2 | 2DL1, 3DL1 | N/A | N/A | N/A |
Given is the internal de-identification code of the NK-cell donors (SNK), stem cell donors (SSC), the donor-specific inhibitory KIR repertoire relevant for characterization of the alloreactive subset, the KIRL repertoire of the respective BCP-ALL, and the identification of the educated KIRs that are specific for the missing KIRL in the target cells. Note that the KIR2DL1 and KIR2DL2/3 KIR family members recognize HLA-C alleles with Lys80 (C2 epitope) or Asn80 (C1 epitope) residues, respectively, whereas KIR3DL1 recognizes HLA-A and B alleles with a Bw4 supertypic specificity, and KIR3DL2 recognizes HLA-A3 and HLA-A11 alleles. For detailed KIR ligand status and KIR expression data, see supplemental Tables 1 and 2.
N/A = not applicable.
Monoallelic data as the NALM-16 cell line has a near-haploid genome.
Note that in contrast to theoretical assumptions, this donor does not express KIR3DL1 on the NK-cell surface and does therefore lack a KIR3DL1+ alloreactive NK-cell subset.
Note that the assumption of KIR3DL1 is based on genomic data, as huNSG mice lacked inhibitory KIR surface expression.
Genomic expression of KIR2DL2 is absent in this donor.