VWF fragment/FVIII binding constants
| VWF fragment . | kon (min−1nM−1) . | koff (min−1) . | KD (nM) . |
|---|---|---|---|
| D1-D3 | 1.7 × 10−2 ± 1.3 × 10−3 | 7.8 × 10−3 ± 7.7 × 10−4 | 0.48 ± 0.066 |
| D′D3 | 1.9 × 10−2 ± 1.3 × 10−3 | 1.8 × 10−2 ± 8.9 × 10−4* | 0.92 ± 0.049* |
| D′D3-Fc | 1.3 × 10−2 ± 1.2 × 10−3 | 1.9 × 10−2 ± 1.8 × 10−3* | 1.5 ± 0.17* |
| truncD′D3 | 1.6 × 10−2 ± 1.3 × 10−3 | 5.5 × 10−2 ± 4.5 × 10−3* | 3.8 ± 0.51* |
| VWF fragment . | kon (min−1nM−1) . | koff (min−1) . | KD (nM) . |
|---|---|---|---|
| D1-D3 | 1.7 × 10−2 ± 1.3 × 10−3 | 7.8 × 10−3 ± 7.7 × 10−4 | 0.48 ± 0.066 |
| D′D3 | 1.9 × 10−2 ± 1.3 × 10−3 | 1.8 × 10−2 ± 8.9 × 10−4* | 0.92 ± 0.049* |
| D′D3-Fc | 1.3 × 10−2 ± 1.2 × 10−3 | 1.9 × 10−2 ± 1.8 × 10−3* | 1.5 ± 0.17* |
| truncD′D3 | 1.6 × 10−2 ± 1.3 × 10−3 | 5.5 × 10−2 ± 4.5 × 10−3* | 3.8 ± 0.51* |
Kinetic constants were determined from 3 to 4 independent binding experiments at 4 FVIII concentrations. Reported values (mean ± standard error of the mean) were averaged from regressions with r2 > 0.95.
KD, dissociation constant = koff/kon; koff, dissociation rate; kon, association rate.
P < .0005 vs D1-D3.