Cellular and exosomal miRNA profiles of U266-HR cells
| Ct value . | miRNA . | (1) Acute hypoxia−, U266-HR↑ . | miRNA . | (2) Acute hypoxia↑, U266-HR↑ . | ||
|---|---|---|---|---|---|---|
| Fold change (vs parental cell) . | Fold change (vs parental cell) . | |||||
| Exosome . | Cell . | Exosome . | Cell . | |||
| 20-25 | miR-193b | 2.0112 | 2.6867 | miR-210 | 17.486 | 33.3568 |
| miR-138 | 2.1346 | 3.1043 | ||||
| 25-30 | miR-328 | 2.1138 | 1.9223 | |||
| let-7e | 1.6542 | 1.8644 | ||||
| miR-204 | 16.0121 | 5.2747 | ||||
| miR-223 | 2.45 | 12.9772 | ||||
| miR-335 | 11.6635 | 21.1083 | ||||
| 30-35 | miR-23a | 2.4124 | 5.4919 | |||
| miR-200c | 3.1872 | 2.8421 | ||||
| miR-185 | 2.4197 | 2.7435 | ||||
| Ct value . | miRNA . | (1) Acute hypoxia−, U266-HR↑ . | miRNA . | (2) Acute hypoxia↑, U266-HR↑ . | ||
|---|---|---|---|---|---|---|
| Fold change (vs parental cell) . | Fold change (vs parental cell) . | |||||
| Exosome . | Cell . | Exosome . | Cell . | |||
| 20-25 | miR-193b | 2.0112 | 2.6867 | miR-210 | 17.486 | 33.3568 |
| miR-138 | 2.1346 | 3.1043 | ||||
| 25-30 | miR-328 | 2.1138 | 1.9223 | |||
| let-7e | 1.6542 | 1.8644 | ||||
| miR-204 | 16.0121 | 5.2747 | ||||
| miR-223 | 2.45 | 12.9772 | ||||
| miR-335 | 11.6635 | 21.1083 | ||||
| 30-35 | miR-23a | 2.4124 | 5.4919 | |||
| miR-200c | 3.1872 | 2.8421 | ||||
| miR-185 | 2.4197 | 2.7435 | ||||
After the cellular and exosomal miRNAs were extracted by a fold change of <1.5 and Ct value, they were subdivided into 2 groups: (1) miRNAs upregulated only in HR-MM cells (Acute hypoxia−, U266-HR↑), and (2) miRNAs upregulated in both acute hypoxia and HR-MM cells (Acute hypoxia↑, U266-HR↑).