Cellular and exosomal miRNA profiles of RPMI8226-HR cells
| Ct value . | miRNA . | (1) Acute hypoxia−, RPMI8226-HR↑ . | miRNA . | (2) Acute hypoxia↑, RPMI8226-HR↑ . | ||
|---|---|---|---|---|---|---|
| Fold change (vs parental cell) . | Fold change (vs parental cell) . | |||||
| Exosome . | Cell . | Exosome . | Cell . | |||
| 20-25 | miR-425 | 2.1558 | 2.4911 | miR-210 | 17.486 | 23.9463 |
| miR-27a | 1.7622 | 2.7311 | ||||
| miR-27b | 2.0682 | 4.4697 | ||||
| miR-223 | 2.671 | 6.33 | ||||
| miR-135a | 16.991 | 18.1049 | ||||
| miR-324-3p | 1.5767 | 1.8605 | ||||
| miR-361-5p | 1.71 | 3.4489 | ||||
| miR-383 | 4.1609 | 10.8108 | ||||
| miR-551b | 3.6555 | 2.5412 | ||||
| 25-30 | miR-140-3p | 2.1908 | 2.5292 | |||
| miR-500 | 2.2111 | 4.8255 | ||||
| miR-135b | 7.9551 | 21.8038 | ||||
| miR-483-5p | 2.5079 | 1.6511 | ||||
| miR-362-5p | 1.747 | 2.7095 | ||||
| Ct value . | miRNA . | (1) Acute hypoxia−, RPMI8226-HR↑ . | miRNA . | (2) Acute hypoxia↑, RPMI8226-HR↑ . | ||
|---|---|---|---|---|---|---|
| Fold change (vs parental cell) . | Fold change (vs parental cell) . | |||||
| Exosome . | Cell . | Exosome . | Cell . | |||
| 20-25 | miR-425 | 2.1558 | 2.4911 | miR-210 | 17.486 | 23.9463 |
| miR-27a | 1.7622 | 2.7311 | ||||
| miR-27b | 2.0682 | 4.4697 | ||||
| miR-223 | 2.671 | 6.33 | ||||
| miR-135a | 16.991 | 18.1049 | ||||
| miR-324-3p | 1.5767 | 1.8605 | ||||
| miR-361-5p | 1.71 | 3.4489 | ||||
| miR-383 | 4.1609 | 10.8108 | ||||
| miR-551b | 3.6555 | 2.5412 | ||||
| 25-30 | miR-140-3p | 2.1908 | 2.5292 | |||
| miR-500 | 2.2111 | 4.8255 | ||||
| miR-135b | 7.9551 | 21.8038 | ||||
| miR-483-5p | 2.5079 | 1.6511 | ||||
| miR-362-5p | 1.747 | 2.7095 | ||||
After the cellular and exosomal miRNAs were extracted by a fold change of <1.5 and Ct value, they were subdivided into 2 groups: (1) miRNAs upregulated only in HR-MM cells (Acute hypoxia−, RPMI8226-HR↑), and (2) miRNAs upregulated in both acute hypoxia and HR-MM cells (Acute hypoxia↑, RPMI8226-HR↑).